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Literature summary extracted from

  • Coleman, K.J.; Cornish-Bowden, A.; Cole, J.A.
    Purification and properties of nitrite reductase from Escherichia coli K12 (1978), Biochem. J., 175, 483-493.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.4 1,10-phenanthroline
-
Escherichia coli
1.7.1.4 2,2'-bipyridine
-
Escherichia coli
1.7.1.4 arsenite 10 mM, 24% inhibition Escherichia coli
1.7.1.4 CN-
-
Escherichia coli
1.7.1.4 NaN3
-
Escherichia coli
1.7.1.4 p-chloromercuribenzoate 0.02 mM, complete inactivation, partially prevented by 10 mM cysteine Escherichia coli
1.7.1.4 SO32- 1 mM, 22% inhibition Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.1.4 cell associated
-
Escherichia coli
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.4 88000
-
2 * 88000, SDS-PAGE Escherichia coli
1.7.1.4 190000
-
glycerol density gradient centrifugation Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.4 NAD(P)H + nitrite + H+ Escherichia coli
-
NAD(P)+ + NH3 + H2O
-
?
1.7.1.4 NAD(P)H + nitrite + H+ Escherichia coli OR75Ch15
-
NAD(P)+ + NH3 + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.4 Escherichia coli
-
-
-
1.7.1.4 Escherichia coli OR75Ch15
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.4 ammonium sulfate, DEAE-cellulose, DEAE-Sephadex Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.4 16.7
-
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.4 NAD(P)H + NH2OH
-
Escherichia coli NAD(P)+ + NH3 + H2O
-
?
1.7.1.4 NAD(P)H + NH2OH
-
Escherichia coli OR75Ch15 NAD(P)+ + NH3 + H2O
-
?
1.7.1.4 NAD(P)H + nitrite + H+
-
Escherichia coli NAD(P)+ + NH3 + H2O
-
?
1.7.1.4 NAD(P)H + nitrite + H+
-
Escherichia coli OR75Ch15 NAD(P)+ + NH3 + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.7.1.4 dimer 2 * 88000, SDS-PAGE Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.4 flavin 0.4 mol of flavin per mol of enzyme Escherichia coli
1.7.1.4 additional information heme is not detectable Escherichia coli
1.7.1.4 NADH
-
Escherichia coli