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Literature summary extracted from

  • Wang, R.; Nicholas, D.J.D.
    Some properties of nitrite and hydroxylamine reductases from Derxia gummosa (1986), Phytochemistry, 25, 2463-2469.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.4 1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide metho-p-toluenesulfonate preincubation with NO2- completely protects Derxia gummosa
1.7.1.4 2,2'-bipyridine 1 mM, 40% inhibition, no inhibition if enzyme is preincubated with nitrite or NH2OH prior to adding the inhibitor Derxia gummosa
1.7.1.4 8-hydroxyquinoline 1 mM, 96% inhibition Derxia gummosa
1.7.1.4 antimycin A 0.2 mM, 45% inhibition Derxia gummosa
1.7.1.4 CN- 1 mM, 45% inhibition, no inhibition if enzyme is preincubated with nitrite or NH2OH prior to adding the inhibitor Derxia gummosa
1.7.1.4 dinitrophenol
-
 Derxia gummosa
1.7.1.4 hydroxylamine competitive vs. nitrite Derxia gummosa
1.7.1.4 Iodine
-
 Derxia gummosa
1.7.1.4 KCNS 0.1 mM, 90% inhibition of NH2OH reductase activity, nitrite reductase activity is not affected Derxia gummosa
1.7.1.4 N-ethylmaleimide 0.1 mM, 52% inhibition Derxia gummosa
1.7.1.4 NH2OH competitive vs. NO2- Derxia gummosa
1.7.1.4 nitrite competitive vs. hydroxylamine Derxia gummosa
1.7.1.4 p-chloromercuribenzoate 0.1 mM, 60% inhibition Derxia gummosa
1.7.1.4 Rose bengal preincubation with NO2- completely protects Derxia gummosa
1.7.1.4 rotenone 0.1 mM, 56% inhibition Derxia gummosa
1.7.1.10 1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide metho-p-toluenesulfonate 1.5 mM, 68% inhibition, hydroxylamine protects Derxia gummosa
1.7.1.10 2,2'-dipyridyl
-
 Derxia gummosa
1.7.1.10 8-hydroxyquinoline 0.1 mM, 50% inhibition of hydroxylamine reductase activity Derxia gummosa
1.7.1.10 Antimycin 1 0.1 mM, 26% inhibition of hydroxylamine reductase activity Derxia gummosa
1.7.1.10 dinitrophenol 0.1 mM, 80% inhibition of hydroxylamine reductase activity Derxia gummosa
1.7.1.10 Iodine 0.5 mM, 80% inhibition, preincubation with hydroxylamine protects Derxia gummosa
1.7.1.10 N-ethylmaleimide 0.1 mM, 58% inhibition of hydroxylamine reductase activity Derxia gummosa
1.7.1.10 NADH inhibits in absence of hydroxylamine Derxia gummosa
1.7.1.10 nitrite nitrite and hydroxylamine reductase activity are associated with the same enzyme, nitrite and hydroxylamine are competitive for the same binding site Derxia gummosa
1.7.1.10 p-chloromercuribenzoate 0.1 mM, 67% inhibition of hydroxylamine reductase activity Derxia gummosa
1.7.1.10 potassium cyanide
-
 Derxia gummosa
1.7.1.10 potassium thiocyanate 0.1 mM, 90% inhibition of hydroxylamine reductase activity, nitrite reductase activity is not inhibited Derxia gummosa
1.7.1.10 Rose bengal 0.5 mM, 94% inhibition Derxia gummosa
1.7.1.10 rotenone 0.1 mM, 52% inhibition of hydroxylamine reductase activity Derxia gummosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.4 0.0048
-
 NO2-
-
 Derxia gummosa
1.7.1.4 0.0063
-
 NADH + NO2- Derxia gummosa
1.7.1.4 0.15
-
 NADH + hydroxylamine Derxia gummosa
1.7.1.4 5.3
-
 hydroxylamine
-
 Derxia gummosa
1.7.1.10 0.0048
-
 NO2-
-
 Derxia gummosa
1.7.1.10 0.15
-
 NADH
-
 Derxia gummosa
1.7.1.10 5.3
-
 hydroxylamine
-
 Derxia gummosa

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.4 Derxia gummosa
-
-
-
1.7.1.10 Derxia gummosa
-
 nitrite and hydroxylamine reductase are associated within the same enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.10 nitrite and hydroxylamine reductase are associated with the same enzyme Derxia gummosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.4 0.422
-
 reduction of NO2- Derxia gummosa
1.7.1.4 0.868
-
 reduction of hydroxylamine Derxia gummosa
1.7.1.10 0.422
-
 reduction of nitrite Derxia gummosa
1.7.1.10 0.68
-
 reduction of hydroxylamine Derxia gummosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.4 NAD(P)H + NH2OH
-
 Derxia gummosa NAD(P)+ + NH3 + H2O
-
 ?
1.7.1.4 NAD(P)H + nitrite + H+ NADH is preferred, reduced benzyl viologen or methyl viologen can act as artificial electron donors Derxia gummosa NAD(P)+ + NH3 + H2O
-
 ?
1.7.1.4 nitrite + NADH + H+
-
 Derxia gummosa NH3 + NAD+ + H2O
-
 r
1.7.1.10 hydroxylamine + NADH 100% activity with NADH, 46% with NADPH, 117% with FADH2, 105% with FMNH2, 220% with methylviologen, 210% with benzyl viologen, 57% with Na2S2O4 Derxia gummosa NH3 + NAD+ + H2O
-
 ?
1.7.1.10 NO2- + NADH enzyme has both nitrite reductase and hydroxylamine reductase activity, main function may be nitrite reduction Derxia gummosa NH3 + H2O + NAD+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.4 8.5
-
-
 Derxia gummosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.4 NADH
-
 Derxia gummosa
1.7.1.4 NADPH
-
 Derxia gummosa
1.7.1.10 NADH
-
 Derxia gummosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7.1.4 0.52
-
 NO2-
-
 Derxia gummosa
1.7.1.4 24
-
 NH2OH
-
 Derxia gummosa
1.7.1.10 0.52
-
 NO2-
-
 Derxia gummosa