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Literature summary extracted from

  • Bray, R.C.
    Molybdenum iron-sulfur flavin hydroxylases and related enzymes (1975), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 12, 299-419.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.3.1 arsenite
-
Bos taurus
1.8.3.1 arsenite
-
Gallus gallus
1.8.3.1 arsenite
-
Oryctolagus cuniculus
1.8.3.1 arsenite
-
Rattus norvegicus
1.8.3.1 arsenite
-
Sus scrofa
1.8.3.1 CN-
-
Bos taurus
1.8.3.1 CN-
-
Gallus gallus
1.8.3.1 CN-
-
Oryctolagus cuniculus
1.8.3.1 CN-
-
Rattus norvegicus
1.8.3.1 CN-
-
Sus scrofa
1.17.3.2 2,4-Diamino-6-hydroxy-s-triazine
-
Bos taurus
1.17.3.2 2,4-dinitrofluorobenzene
-
Bos taurus
1.17.3.2 2-Amino-4-hydroxypterine-6-aldehyde
-
Bos taurus
1.17.3.2 allopurinol
-
Bos taurus
1.17.3.2 arsenite
-
Bos taurus
1.17.3.2 cyanide
-
Bos taurus
1.17.3.2 cyanide
-
Gallus gallus
1.17.3.2 formaldehyde
-
Bos taurus
1.17.3.2 guanine
-
Bos taurus
1.17.3.2 methanol
-
Bos taurus
1.17.3.2 methanol
-
Gallus gallus
1.17.3.2 Purine-6-aldehyde
-
Bos taurus
1.17.3.2 salicylate
-
Bos taurus
1.17.3.2 thiocyanate
-
Drosophila melanogaster
1.17.3.2 thiocyanate
-
Gallus gallus
1.17.3.2 Urea
-
Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.3.1 0.002
-
cytochrome c
-
Gallus gallus
1.8.3.1 0.002
-
cytochrome c
-
Rattus norvegicus
1.8.3.1 0.39
-
sulfite
-
Bos taurus
1.17.3.2 additional information
-
additional information
-
Drosophila melanogaster
1.17.3.2 additional information
-
additional information
-
Bos taurus
1.17.3.2 0.05
-
O2 cosubstrate xanthine, pH 8.5 Bos taurus
1.17.3.2 0.08
-
O2 cosubstrate xanthine, pH 10.0 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.3.1 mitochondrion
-
Gallus gallus 5739
-
1.8.3.1 mitochondrion
-
Rattus norvegicus 5739
-
1.8.3.1 mitochondrion
-
Sus scrofa 5739
-
1.8.3.1 mitochondrion
-
Bos taurus 5739
-
1.8.3.1 mitochondrion
-
Oryctolagus cuniculus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.3.1 Mo
-
Gallus gallus
1.8.3.1 Mo
-
Rattus norvegicus
1.8.3.1 Mo
-
Sus scrofa
1.8.3.1 Mo
-
Bos taurus
1.8.3.1 Mo
-
Oryctolagus cuniculus
1.17.3.2 Iron iron-molybdenum protein Gallus gallus
1.17.3.2 Iron iron-molybdenum protein Drosophila melanogaster
1.17.3.2 Iron iron-molybdenum protein Bos taurus
1.17.3.2 Iron contains FAD, iron and molybdenum in the ratio 1/4/4 Gallus gallus
1.17.3.2 Iron contains FAD, iron and molybdenum in the ratio 1/4/4 Drosophila melanogaster
1.17.3.2 Iron contains FAD, iron and molybdenum in the ratio 1/4/4 Bos taurus
1.17.3.2 Molybdenum contains FAD, molybdenum and iron in the ratio 1/4/4 Gallus gallus
1.17.3.2 Molybdenum contains FAD, molybdenum and iron in the ratio 1/4/4 Drosophila melanogaster
1.17.3.2 Molybdenum contains FAD, molybdenum and iron in the ratio 1/4/4 Bos taurus
1.17.3.2 Molybdenum an iron-molybdenum protein Gallus gallus
1.17.3.2 Molybdenum an iron-molybdenum protein Drosophila melanogaster
1.17.3.2 Molybdenum an iron-molybdenum protein Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.3.2 additional information
-
-
Gallus gallus
1.17.3.2 additional information
-
-
Bos taurus
1.17.3.2 120000
-
2 * 120000 Gallus gallus
1.17.3.2 150000
-
2 * 150000, SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.1 Bos taurus
-
-
-
1.8.3.1 Gallus gallus
-
-
-
1.8.3.1 Oryctolagus cuniculus
-
-
-
1.8.3.1 Rattus norvegicus
-
-
-
1.8.3.1 Sus scrofa
-
-
-
1.17.3.2 Bos taurus
-
-
-
1.17.3.2 Drosophila melanogaster
-
-
-
1.17.3.2 Gallus gallus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.17.3.2 additional information
-
Gallus gallus
1.17.3.2 additional information
-
Drosophila melanogaster
1.17.3.2 additional information
-
Bos taurus
1.17.3.2 proteolytic modification
-
Gallus gallus
1.17.3.2 proteolytic modification
-
Drosophila melanogaster
1.17.3.2 proteolytic modification
-
Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.3.1
-
Gallus gallus
1.8.3.1
-
Rattus norvegicus
1.8.3.1
-
Sus scrofa
1.8.3.1
-
Bos taurus
1.8.3.1
-
Oryctolagus cuniculus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.17.3.2 xanthine + H2O + O2 = urate + H2O2 mechanism Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.3.1 additional information
-
Gallus gallus
-
1.8.3.1 additional information
-
Rattus norvegicus
-
1.8.3.1 additional information
-
Sus scrofa
-
1.8.3.1 additional information
-
Oryctolagus cuniculus
-
1.17.3.2 liver
-
Gallus gallus
-
1.17.3.2 liver
-
Bos taurus
-
1.17.3.2 milk
-
Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.3.1 additional information
-
-
Gallus gallus
1.8.3.1 additional information
-
-
Rattus norvegicus
1.8.3.1 additional information
-
-
Sus scrofa
1.8.3.1 additional information
-
-
Bos taurus
1.8.3.1 additional information
-
-
Oryctolagus cuniculus
1.17.3.2 2.4 5.1
-
Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.1 sulfite + cytochrome c
-
Gallus gallus sulfate + reduced cytochrome c
-
?
1.8.3.1 sulfite + cytochrome c
-
Rattus norvegicus sulfate + reduced cytochrome c
-
?
1.8.3.1 sulfite + H2O + A
-
Bos taurus SO42- + AH2
-
?
1.17.3.2 1-methyl-2-hydroxypurine + H2O + O2
-
Bos taurus 1-methyl-2-hydroxy-7,9-dihydropurin-8-one + H2O2
-
?
1.17.3.2 1-methylxanthine + H2O + O2
-
Bos taurus 1-methylurate + H2O2
-
?
1.17.3.2 3-methylhypoxanthine + H2O + O2
-
Bos taurus 3-methylxanthine + H2O2
-
?
1.17.3.2 6,8-dihydroxypurine + H2O + O2
-
Bos taurus ?
-
?
1.17.3.2 6-cyanopurine + H2O + O2
-
Bos taurus 6-cyano-7,9-dihydropurine-8-one
-
?
1.17.3.2 6-thioxanthine + H2O + O2
-
Bos taurus 6-thiourate + H2O2
-
?
1.17.3.2 acetaldehyde + H2O + O2
-
Bos taurus acetic acid + H2O2
-
?
1.17.3.2 adenine + H2O + O2
-
Bos taurus 6-amino-7,9-dihydropurin-8-one + H2O2
-
?
1.17.3.2 allopurinol + H2O + O2
-
Bos taurus ?
-
?
1.17.3.2 hypoxanthine + 2 H2O + 2 O2
-
Gallus gallus urate + 2 H2O2
-
?
1.17.3.2 hypoxanthine + 2 H2O + 2 O2
-
Drosophila melanogaster urate + 2 H2O2
-
?
1.17.3.2 hypoxanthine + 2 H2O + 2 O2
-
Bos taurus urate + 2 H2O2
-
?
1.17.3.2 N1-methylnicotinamide + H2O + O2
-
Bos taurus ?
-
?
1.17.3.2 NADH + H2O + O2
-
Bos taurus NAD+ + H2O2
-
?
1.17.3.2 pteridine + H2O + O2 and derivatives, e.g.: 4-amino-7-hydroxy pteridine, 4-hydroxy-7-azapteridine Bos taurus ?
-
?
1.17.3.2 purine + H2O + O2 purine and derivatives Bos taurus 7,9-dihydropurin-8-one + H2O2
-
?
1.17.3.2 pyrimidine derivatives + H2O + O2 e.g. 2-hydroxypyrimidine Bos taurus ?
-
?
1.17.3.2 pyrimidine derivatives + H2O + O2 6-hydroxy-6-aminepyrimidine Bos taurus ?
-
?
1.17.3.2 pyrimidine derivatives + H2O + O2 7-hydroxy-(1,2,5)-thiadiazolo(3,4-d)-pyrimidine Gallus gallus ?
-
?
1.17.3.2 pyrimidine derivatives + H2O + O2 7-hydroxy-(1,2,5)-thiadiazolo(3,4-d)-pyrimidine Drosophila melanogaster ?
-
?
1.17.3.2 salicylaldehyde + H2O + O2
-
Bos taurus salicylic acid + H2O2
-
?
1.17.3.2 xanthine + H2O + O2 electron acceptor methylene blue Bos taurus uric acid + H2O2 under some conditions the product is mainly superoxide rather than peroxide: RH + H2O + 2 O2 = ROH + 2 H+ + 2 O2- ?
1.17.3.2 xanthine + H2O + O2 electron acceptor NAD+ Bos taurus uric acid + H2O2 under some conditions the product is mainly superoxide rather than peroxide: RH + H2O + 2 O2 = ROH + 2 H+ + 2 O2- ?
1.17.3.2 xanthine + H2O + O2 electron acceptor O2 Gallus gallus uric acid + H2O2
-
?
1.17.3.2 xanthine + H2O + O2 electron acceptor O2 Drosophila melanogaster uric acid + H2O2
-
?
1.17.3.2 xanthine + H2O + O2 electron acceptor O2 Bos taurus uric acid + H2O2 under some conditions the product is mainly superoxide rather than peroxide: RH + H2O + 2 O2 = ROH + 2 H+ + 2 O2- ?
1.17.3.2 xanthine + H2O + O2 electron acceptor quinones Bos taurus uric acid + H2O2 under some conditions the product is mainly superoxide rather than peroxide: RH + H2O + 2 O2 = ROH + 2 H+ + 2 O2- ?
1.17.3.2 xanthine + H2O + O2 specificity for electron acceptor is low Bos taurus uric acid + H2O2 under some conditions the product is mainly superoxide rather than peroxide: RH + H2O + 2 O2 = ROH + 2 H+ + 2 O2- ?
1.17.3.2 xanthine + H2O + O2 enzyme also oxidizes hypoxanthine, some other purines, pterines and aldehydes, i.e. possesses the activity of EC 1.2.3.1, probably acts on the hydrated derivatives of these substrates Bos taurus uric acid + H2O2 under some conditions the product is mainly superoxide rather than peroxide: RH + H2O + 2 O2 = ROH + 2 H+ + 2 O2- ?
1.17.3.2 xanthine + H2O + O2 low specificity to substrate Bos taurus uric acid + H2O2 under some conditions the product is mainly superoxide rather than peroxide: RH + H2O + 2 O2 = ROH + 2 H+ + 2 O2- ?
1.17.3.2 xanthine + H2O + O2 electron acceptor ferricyanide Bos taurus uric acid + H2O2 under some conditions the product is mainly superoxide rather than peroxide: RH + H2O + 2 O2 = ROH + 2 H+ + 2 O2- ?
1.17.3.2 xanthine + H2O + O2 electron acceptor 2,6-dichlorophenolindophenol Bos taurus uric acid + H2O2 under some conditions the product is mainly superoxide rather than peroxide: RH + H2O + 2 O2 = ROH + 2 H+ + 2 O2- ?

Subunits

EC Number Subunits Comment Organism
1.17.3.2 dimer 2 * 150000, SDS-PAGE Bos taurus
1.17.3.2 dimer 2 * 130000-140000 Drosophila melanogaster
1.17.3.2 dimer 2 * 120000 Gallus gallus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.17.3.2 16.2
-
xanthine pH 8.2, 23.5°C Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.3.1 8.6
-
with O2 as acceptor Bos taurus
1.17.3.2 8.3
-
-
Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.3.1 heme
-
Gallus gallus
1.8.3.1 heme
-
Rattus norvegicus
1.8.3.1 heme
-
Sus scrofa
1.8.3.1 heme
-
Bos taurus
1.8.3.1 heme
-
Oryctolagus cuniculus
1.17.3.2 FAD flavoprotein Gallus gallus
1.17.3.2 FAD flavoprotein Drosophila melanogaster
1.17.3.2 FAD flavoprotein Bos taurus
1.17.3.2 FAD contains FAD, molybdenum and iron in the ratio 1 : 4 : 4 Gallus gallus
1.17.3.2 FAD contains FAD, molybdenum and iron in the ratio 1 : 4 : 4 Drosophila melanogaster
1.17.3.2 FAD contains FAD, molybdenum and iron in the ratio 1 : 4 : 4 Bos taurus