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Literature summary extracted from

  • Arend, J.; Warzecha, H.; Hefner, T.; Stöckigt, J.
    Utilizing genetically engineered bacteria to produce plant-specific glucosides (2001), Biotechnol. Bioeng., 76, 126-131.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.218 synthesis formation of arbutin, which is a potent inhibitor of human melanin biosynthesis with commercial value Rauvolfia serpentina

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.218 expression in Escherichia coli Rauvolfia serpentina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.218 additional information
-
 additional information Km value for hydroquinone is below 0.001 mM Rauvolfia serpentina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.218 51800
-
 x * 51800, calculation from nucleotide sequence Rauvolfia serpentina

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.218 Rauvolfia serpentina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.218
-
 Rauvolfia serpentina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.218 UDP-glucose + 4-hydroxyacetophenone ,1.8% of the activity with hydroquinone Rauvolfia serpentina UDP + (4-acetophenyl)-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + coniferyl alcohol 1.2% of the activity with hydroquinone Rauvolfia serpentina UDP + coniferin
-
 ?
2.4.1.218 UDP-glucose + eugenol 6.2% of the activity with hydroquinone Rauvolfia serpentina UDP + (4-allyl-2-methoxyphenyl)-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + hydroquinone
-
 Rauvolfia serpentina UDP + hydroquinone-O-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + methylvanillate 2.2% of the activity with hydroquinone Rauvolfia serpentina UDP + 4-(beta-D-glucopyranosyloxy)-3-methoxybenzoic acid
-
 ?
2.4.1.218 UDP-glucose + phenol 5.1% of the activity with hydroquinone Rauvolfia serpentina UDP + phenyl-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + resorcinol 8.7% of the activity with hydroquinone Rauvolfia serpentina UDP + (3-hydroxyphenyl)-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + saligenin 9.4% of the activity with hydroquinone Rauvolfia serpentina UDP + 2-(hydroxymethyl)phenyl beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + thymol 7.6% of the activity with hydroquinone Rauvolfia serpentina UDP + (2-isopropyl-5-methyl-phenyl)-beta-D-glucopyranoside
-
 ?
2.4.1.218 UDP-glucose + vanillic acid 1.0% of the activity with hydroquinone Rauvolfia serpentina UDP + 4-(beta-D-glucopyranosyloxy)-3-methoxybenzoic acid
-
 ?
2.4.1.218 UDP-glucose + vanillin 9.4% of the activity with hydroquinone Rauvolfia serpentina UDP + 4-beta-D-glucopyranosyloxy-3-methoxy-benzaldehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.218 ? x * 51800, calculation from nucleotide sequence Rauvolfia serpentina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.218 50
-
-
 Rauvolfia serpentina