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Literature summary extracted from

  • Kraus, P.F.X.; Kutchan, T.M.
    Molecular cloning and heterologous expression of a cDNA encoding berbamunine synthase, a C-O phenol-coupling cytochrome P450 from the higher plant Berberis stolonifera (1995), Proc. Natl. Acad. Sci. USA, 92, 2071-2075.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.66 expression in functional form in insect cell culture using a baculovirus-based expression system Berberis stolonifera
1.14.19.66 expression in Sf9 cells Berberis stolonifera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.19.66 additional information
-
 additional information normal Michaelis-Menten kinetics are not observed for the formation of guattegaumerine or berbamunine Berberis stolonifera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.19.66 46000
-
 x * 46000, SDS-PAGE Berberis stolonifera
1.14.19.66 55474
-
 x * 55474, calculation from nucleotide sequence Berberis stolonifera

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.66 Berberis stolonifera P47195
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.19.66 heterologously expressed enzyme from Sf9 cell culture Berberis stolonifera

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.66 cell suspension culture
-
 Berberis stolonifera
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.66 2 (S)-N-methylcoclaurine + 2 (R)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
-
 Berberis stolonifera berbamunine + guattegaumerine + [oxidized NADPH-hemoprotein reductase] + H2O two molecules of (R)-N-methylcoclaurine for the (R,R) dimer guattegaumerine or one molecule each of (R)-N-methylcoclaurine and (S)-N-methylcoclaurine form the (R,S) dimer berbamunine. The ratio of the two bisbenzylisoquinolines formed can be altered by the reductase source or by varying the enantiomer composition of the substrates ?

Subunits

EC Number Subunits Comment Organism
1.14.19.66 ? x * 46000, SDS-PAGE Berberis stolonifera
1.14.19.66 ? x * 55474, calculation from nucleotide sequence Berberis stolonifera

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.19.66 35
-
 formation of berbamunine Berberis stolonifera
1.14.19.66 38
-
 formation of guattegaumerine Berberis stolonifera

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.19.66 7 49 50% of maximal activity at 7°C and 49°C, formation of berbamunine Berberis stolonifera
1.14.19.66 18 46 50% of maximal activity at 18°C and 46°C, formation of guattegaumerine Berberis stolonifera

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.19.66 additional information
-
 additional information
-
 Berberis stolonifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.19.66 7.5
-
 formation of berbamunine or guattegaumerine Berberis stolonifera