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Literature summary extracted from

  • Sorensen, S.H.; Noren, O.; Sjoestroem, H.; Danielsen, E.M.
    Amphiphilic pig intestinal microvillus maltase/glucoamylase. Structure and specificity (1982), Eur. J. Biochem., 126, 559-568.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.20 repeated freezing and thawing inactivates Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 1.74
-
 maltose
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 210000
-
 enzyme solubilized by papain, gel filtration Sus scrofa
3.2.1.20 330000
-
 enzyme solubilized by Triton X-100, gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.20 intestine microvillus Sus scrofa
-
3.2.1.20 pancreas
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 additional information
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
3.2.1.20 4°C, stable for 1 month Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 amylopectin + H2O
-
 Sus scrofa alpha-D-glucose
-
 ?
3.2.1.20 isopanose + H2O
-
 Sus scrofa alpha-D-glucose
-
 ?
3.2.1.20 kojibiose + H2O
-
 Sus scrofa alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltohexaose + H2O
-
 Sus scrofa maltopentaose + D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 Sus scrofa alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltotetraose + H2O
-
 Sus scrofa alpha-D-glucose
-
 ?
3.2.1.20 maltotriose + H2O
-
 Sus scrofa maltose + D-glucose
-
 ?
3.2.1.20 nigerose + H2O
-
 Sus scrofa 2 alpha-D-glucose
-
 ?
3.2.1.20 starch + H2O
-
 Sus scrofa alpha-D-glucose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 6 7
-
 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.20 4.5 9 pH 4.5: about 55% of activity with maltose as substrate, about 30% of activity with starch as substrate, pH 8.0: about 60% of activity with maltose as substrate, about 50% of activity with starch as substrate Sus scrofa