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Literature summary extracted from
- Characterization of high pI alpha-glucosidase from germinated barley seeds: substrate specificity subsite affinities and active-site residues (1995), Carbohydr. Res., 277, 145-159.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.20 | conduritol B epoxide | - |
Hordeum vulgare |  |
| 3.2.1.20 | D-glucose | hydrolysis of p-nitrophenyl alpha-glucoside | Hordeum vulgare | |
| 3.2.1.20 | N-bromosuccinimide | - |
Hordeum vulgare | |
| 3.2.1.20 | Phenylglyoxal | - |
Hordeum vulgare | |
| 3.2.1.20 | pyridoxal 5'-phosphate | - |
Hordeum vulgare | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.20 | 1.04 | - |
p-nitrophenyl alpha-glucoside | - |
Hordeum vulgare | |
| 3.2.1.20 | 1.11 | - |
maltotriose | - |
Hordeum vulgare | |
| 3.2.1.20 | 1.29 | - |
nigerose | - |
Hordeum vulgare | |
| 3.2.1.20 | 1.91 | - |
maltose | - |
Hordeum vulgare | |
| 3.2.1.20 | 2.37 | - |
maltotetraose | - |
Hordeum vulgare | |
| 3.2.1.20 | 2.92 | - |
maltopentaose | - |
Hordeum vulgare | |
| 3.2.1.20 | 5.32 | - |
isomaltose | - |
Hordeum vulgare | |
| 3.2.1.20 | 5.44 | - |
maltohexaose | - |
Hordeum vulgare | |
| 3.2.1.20 | 7.89 | - |
maltoheptaose | - |
Hordeum vulgare | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.20 | Hordeum vulgare | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.20 | isomaltose + H2O | - |
Hordeum vulgare | 2 alpha-D-glucose | - |
? | |
| 3.2.1.20 | maltoheptaose + H2O | - |
Hordeum vulgare | maltohexaose + D-glucose | - |
? | |
| 3.2.1.20 | maltohexaose + H2O | - |
Hordeum vulgare | maltopentaose + D-glucose | - |
? | |
| 3.2.1.20 | maltopentaose + H2O | - |
Hordeum vulgare | alpha-D-glucose + ? | - |
? | |
| 3.2.1.20 | maltose + H2O | - |
Hordeum vulgare | alpha-D-glucose + D-glucose | - |
? | |
| 3.2.1.20 | maltotetraose + H2O | - |
Hordeum vulgare | alpha-D-glucose | - |
? | |
| 3.2.1.20 | maltotriose + H2O | - |
Hordeum vulgare | maltose + D-glucose | - |
? | |
| 3.2.1.20 | nigerose + H2O | - |
Hordeum vulgare | 2 alpha-D-glucose | - |
? | |
| 3.2.1.20 | p-nitrophenyl-alpha-D-glucopyranoside + H2O | - |
Hordeum vulgare | 4-nitrophenol + D-glucose | - |
? | |
| 3.2.1.20 | trehalose + H2O | no activity | Hordeum vulgare | alpha-D-glucose | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.20 | 6.02 | - |
maltose | - |
Hordeum vulgare | |
| 3.2.1.20 | 6.26 | - |
maltohexaose | - |
Hordeum vulgare | |
| 3.2.1.20 | 6.65 | - |
maltotriose | - |
Hordeum vulgare | |
| 3.2.1.20 | 6.67 | - |
maltoheptaose | - |
Hordeum vulgare | |
| 3.2.1.20 | 6.98 | - |
maltotetraose | - |
Hordeum vulgare | |
| 3.2.1.20 | 7.15 | - |
maltopentaose | - |
Hordeum vulgare | |
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Release 2023.1 (January 2023)
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