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Literature summary extracted from

  • Sanda, S.; Leustek, T.; Theisen, M.J.; Garavito, R.M.; Benning, C.
    Recombinant Arabidopsis SQD1 converts UDP-glucose and sulfite to the sulfolipid head group precursor UDP-sulfoquinovose in vitro (2001), J. Biol. Chem., 276, 3941-3946.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.13.1.1 additional information sulfate, APS, PAPS, sulfite, thiosulfate, sufide or sulfoglutathione are tested to determine whether they could stimulate the formation of UDP-sulfoquinovose Arabidopsis thaliana

Application

EC Number Application Comment Organism
3.13.1.1 medicine sulfolipids, in which synthesis the enzyme is involved, promise anti-tumor and anti-HIV therapeutics based on their inhibition of DNA polymerase and reverse transcriptase Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.13.1.1 expressed in Escherichia coli Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
3.13.1.1 T145A greatly reduced activity, site-directed mutagenesis, plays critical role for catalytic activity Arabidopsis thaliana
3.13.1.1 T145A mutant shows greatly reduced activity Arabidopsis sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.13.1.1 sulfite increasing concentrations beyond 0.1 mM Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.13.1.1 0.01
-
 sulfite
-
 Arabidopsis thaliana
3.13.1.1 0.01
-
 sulfite
-
 Arabidopsis sp.
3.13.1.1 0.15
-
 UDP-glucose
-
 Arabidopsis thaliana
3.13.1.1 0.15
-
 UDP-glucose
-
 Arabidopsis sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.13.1.1 chloroplast thylakoid Arabidopsis thaliana 9507
-
3.13.1.1 membrane photosynthetic Arabidopsis thaliana 16020
-
3.13.1.1 thylakoid
-
 Arabidopsis thaliana 9579
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.13.1.1 45500
-
 recombinant protein, lacking the transit peptide Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.13.1.1 UDP-glucose + sulfite Arabidopsis thaliana sulfolipid biosynthesis UDP-sulfoquinovose + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.13.1.1 Arabidopsis sp.
-
-
-
3.13.1.1 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.13.1.1 Ni2+-nitrilotriacetic acid column Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.13.1.1 0.0026
-
-
 Arabidopsis thaliana

Storage Stability

EC Number Storage Stability Organism
3.13.1.1 -20°C, 25 mM NaH2PO4, pH 7.5, 300 mM NaCl, 20% glycerol Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.1.1 UDP-glucose + sulfite
-
 Arabidopsis thaliana UDP-sulfoquinovose + H2O
-
 ?
3.13.1.1 UDP-glucose + sulfite sulfolipid biosynthesis Arabidopsis thaliana UDP-sulfoquinovose + H2O
-
 ?
3.13.1.1 UDP-glucose + sulfite
-
 Arabidopsis sp. UDP-6-sulfoquinovose + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
3.13.1.1 dimer
-
 Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.13.1.1 SQD1
-
 Arabidopsis sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.13.1.1 37
-
 assay at Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.13.1.1 0.00167
-
 UDP-glucose
-
 Arabidopsis thaliana
3.13.1.1 0.1
-
 UDP-glucose
-
 Arabidopsis sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.13.1.1 7.5 9.5
-
 Arabidopsis thaliana
3.13.1.1 9
-
-
 Arabidopsis sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.13.1.1 7 10 pH 7.0: about 50% of maximal activity, pH 10.0: about 35% of maximal activity Arabidopsis sp.

Cofactor

EC Number Cofactor Comment Organism Structure
3.13.1.1 NAD+
-
 Arabidopsis thaliana