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Literature summary extracted from

  • Krishnan, T.; Chandra, A.K.
    Purification and characterization of alpha-amylase from Bacillus licheniformis CUMC305 (1983), Appl. Environ. Microbiol., 46, 430-437.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 Ag+
-
 Bacillus licheniformis
3.2.1.1 Al3+
-
 Bacillus licheniformis
3.2.1.1 Cd2+
-
 Bacillus licheniformis
3.2.1.1 Co2+
-
 Bacillus licheniformis
3.2.1.1 Cu2+
-
 Bacillus licheniformis
3.2.1.1 EDTA resistant at 30°C, inhibition at 90°C, inhibition is partially recovered by Cu2+ or Fe2+ Bacillus licheniformis
3.2.1.1 Fe2+
-
 Bacillus licheniformis
3.2.1.1 Hg2+ partially reversed by Cys Bacillus licheniformis
3.2.1.1 iodoacetate around 100 mM Bacillus licheniformis
3.2.1.1 Mn2+
-
 Bacillus licheniformis
3.2.1.1 Ni2+
-
 Bacillus licheniformis
3.2.1.1 PCMB
-
 Bacillus licheniformis
3.2.1.1 Zn2+
-
 Bacillus licheniformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 additional information
-
 additional information
-
 Bacillus licheniformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 Ca2+ stimulates Bacillus licheniformis
3.2.1.1 F- stimulates Bacillus licheniformis
3.2.1.1 Mg2+ stimulates Bacillus licheniformis
3.2.1.1 MoO42- 10-100 mM, stimulates Bacillus licheniformis
3.2.1.1 Na+ stimulates Bacillus licheniformis
3.2.1.1 PO43- 100 mM, stimulates Bacillus licheniformis
3.2.1.1 S2O32- stimulates Bacillus licheniformis
3.2.1.1 SO32- stimulates Bacillus licheniformis
3.2.1.1 SO42- stimulates Bacillus licheniformis
3.2.1.1 WO42- stimulates Bacillus licheniformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 28000
-
 x * 28000, SDS-PAGE Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Bacillus licheniformis
-
-
-
3.2.1.1 Bacillus licheniformis CUMC305
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1
-
 Bacillus licheniformis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 additional information
-
-
 Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 amylopectin + H2O
-
 Bacillus licheniformis additional information
-
 ?
3.2.1.1 amylopectin + H2O
-
 Bacillus licheniformis CUMC305 additional information
-
 ?
3.2.1.1 amylose + H2O
-
 Bacillus licheniformis additional information
-
 ?
3.2.1.1 amylose + H2O
-
 Bacillus licheniformis CUMC305 additional information
-
 ?
3.2.1.1 glycogen + H2O
-
 Bacillus licheniformis additional information
-
 ?
3.2.1.1 glycogen + H2O
-
 Bacillus licheniformis CUMC305 additional information
-
 ?
3.2.1.1 starch + H2O soluble starch Bacillus licheniformis additional information
-
 ?
3.2.1.1 starch + H2O soluble starch Bacillus licheniformis CUMC305 additional information
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 ? x * 28000, SDS-PAGE Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 90
-
-
 Bacillus licheniformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 60
-
 3 h, in absence of substrate, 9% loss of activity Bacillus licheniformis
3.2.1.1 70
-
 3 h, in absence of substrate, 21% loss of activity Bacillus licheniformis
3.2.1.1 80
-
 90 min, in absence of substrate, 29% loss of activity Bacillus licheniformis
3.2.1.1 100
-
 4 h, in presence of starch, completely stable Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 9
-
-
 Bacillus licheniformis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.1 3
-
 30°C, 18 h, 50% loss of activity Bacillus licheniformis
3.2.1.1 4
-
 30°C, 18 h, 34% loss of activity Bacillus licheniformis
3.2.1.1 5
-
 30°C, 18 h, 25% loss of activity Bacillus licheniformis
3.2.1.1 6
-
 30°C, 18 h, 15% loss of activity Bacillus licheniformis
3.2.1.1 7 9 30°C, 18 h, stable Bacillus licheniformis