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Literature summary extracted from

  • Fujino, T.; Sukhumavasi, J.; Sasaki, T.; Ohmiya, K.; Shimizu, S.
    Purification and properties of an endo-1,4-beta-glucanase from Clostridium josui (1989), J. Bacteriol., 171, 4076-4079.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.4 Cysteine hydrochloride slight activation Ruminiclostridium josui
3.2.1.4 dithiothreitol slight activation Ruminiclostridium josui

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 Ca2+ 1 mM, slight inhibition Ruminiclostridium josui
3.2.1.4 Cu2+ CuCl2 Ruminiclostridium josui
3.2.1.4 glutathione slight Ruminiclostridium josui
3.2.1.4 Hg2+ HgCl2 Ruminiclostridium josui

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 6.7
-
 cellopentaose
-
 Ruminiclostridium josui
3.2.1.4 15.4
-
 cellotetraose
-
 Ruminiclostridium josui

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 KCl slight activation Ruminiclostridium josui
3.2.1.4 ZnCl2 slight activation Ruminiclostridium josui

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 45000
-
 x * 45000, SDS-PAGE Ruminiclostridium josui

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Ruminiclostridium josui
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Ruminiclostridium josui

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.4 additional information stationary phase supernatant Ruminiclostridium josui
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 additional information
-
-
 Ruminiclostridium josui

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 avicel + H2O
-
 Ruminiclostridium josui ?
-
 ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Ruminiclostridium josui additional information
-
 ?
3.2.1.4 cellohexaose + H2O
-
 Ruminiclostridium josui additional information cellotriose + cellobiose + cellotetraose ?
3.2.1.4 cellopentaose + H2O
-
 Ruminiclostridium josui additional information cellobiose + cellotriose ?
3.2.1.4 cellotetraose + H2O
-
 Ruminiclostridium josui 2 cellobiose
-
 ?
3.2.1.4 cellotriose + H2O no activity Ruminiclostridium josui D-glucose + cellobiose
-
 ?
3.2.1.4 cellotriose + H2O no reaction Ruminiclostridium josui D-glucose + cellobiose
-
 ?
3.2.1.4 additional information no activity with cellobiose Ruminiclostridium josui ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 45000, SDS-PAGE Ruminiclostridium josui

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 60
-
 hydrolysis of carboxymethylcellulose Ruminiclostridium josui

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 50
-
 1 h, more than 80% of the initial activity is retained Ruminiclostridium josui

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6.8
-
 hydrolysis of carboxymethylcellulose Ruminiclostridium josui