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Literature summary extracted from

  • Fujino, T.; Ohmiya, K.
    Cloning of the celB gene encoding endo-1,4-beta-glucanase-2 from Clostridium josui in Escherichia coli and the properties of the translated product (1991), J. Ferment. Bioeng., 72, 422-425.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Escherichia coli Ruminiclostridium josui

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 42000
-
 x * 42000, recombinant enzyme, SDS-PAGE Ruminiclostridium josui

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Ruminiclostridium josui
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 additional information
-
-
 Ruminiclostridium josui

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 cellohexaose + H2O
-
 Ruminiclostridium josui additional information
-
 ?
3.2.1.4 cellopentaose + H2O
-
 Ruminiclostridium josui additional information cellobiose + cellotriose ?
3.2.1.4 cellotetraose + H2O
-
 Ruminiclostridium josui 2 cellobiose
-
 ?
3.2.1.4 cellotriose + H2O no reaction Ruminiclostridium josui D-glucose + cellobiose
-
 ?
3.2.1.4 additional information no activity with cellobiose Ruminiclostridium josui ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 42000, recombinant enzyme, SDS-PAGE Ruminiclostridium josui

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 60
-
 recombinant enzyme Ruminiclostridium josui

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5.5
-
 recombinant enzyme Ruminiclostridium josui