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Literature summary extracted from

  • DeGaspari, R.; Li, S.C.; Li, Y.T.
    A GM2-specific beta-hexosaminidase from the roe of striped mullet (Mugil cephalus) (1988), J. Biol. Chem., 263, 1325-1328.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.52 low molecular weight protein restores the enzyme's ability to hydrolyze GM2 in high ionic strength Mugil cephalus

General Stability

EC Number General Stability Organism
3.2.1.52 high purification results in great loss of activity Mugil cephalus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 high ionic strength above 0.1 no cleavage of GM2 Mugil cephalus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 170000
-
 beta-hexosaminidase II + III Mugil cephalus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O Mugil cephalus
-
 N-acetyl-D-hexosamines
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Mugil cephalus
-
 striped mullet
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 beta-hexosaminidase II: 202fold , beta-hexosaminidase III: 426fold, still very heterogenous Mugil cephalus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 roe
-
 Mugil cephalus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 21.3
-
-
 Mugil cephalus

Storage Stability

EC Number Storage Stability Organism
3.2.1.52 -20°C, in 10 mM sodium acetate buffer pH 4.0, very unstable Mugil cephalus
3.2.1.52 -20°C, in 5-50 mM sodium citrate buffer pH 4.0, very stable, even after repeated freeze-thawing Mugil cephalus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 GM2 ganglioside + H2O
-
 Mugil cephalus GM3 ganglioside + N-acetyl-beta-D-galactosamine
-
 ?
3.2.1.52 N-acetyl-beta-D-hexosaminides + H2O
-
 Mugil cephalus N-acetyl-D-hexosamines
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
-
 Mugil cephalus 2-acetamido-2-deoxy-D-galactose + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
-
 Mugil cephalus 2-acetamido-2-deoxy-D-glucose + p-nitrophenol
-
 ?
3.2.1.52 Sda oligosaccharide + H2O
-
 Mugil cephalus N-acetyl-D-galactosamine
-
 ?
3.2.1.52 SRO oligosaccharide + H2O
-
 Mugil cephalus N-acetyl-D-galactosamine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 4
-
 substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside Mugil cephalus