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Literature summary extracted from

  • Goda, M.; Hashimoto, Y.; Shimizu, S.; Kobayashi, M.
    Discovery of a novel enzyme, isonitrile hydratase, involved in nitrogen-carbon triple bond cleavage (2001), J. Biol. Chem., 276, 23480-23485.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.103 AgNO3 1 mM, 99% inhibition Pseudomonas putida
4.2.1.103 ammonium persulfate 1 mM, 23% inhibition Pseudomonas putida
4.2.1.103 CdCl2 1 mM, 87% inhibition Pseudomonas putida
4.2.1.103 CoCl2 1 mM, 98% inhibition Pseudomonas putida
4.2.1.103 CuSO4 1 mM, 98% inhibition Pseudomonas putida
4.2.1.103 Diethyldithiocarbamic acid sodium salt 1 mM, 20% inhibition Pseudomonas putida
4.2.1.103 FeSO4 1 mM, 50% inhibition Pseudomonas putida
4.2.1.103 H2O2 1 mM, 49% inhibition Pseudomonas putida
4.2.1.103 HgCl2 1 mM, 99% inhibition Pseudomonas putida
4.2.1.103 hydroxylamine 1 mM, 10% inhibition Pseudomonas putida
4.2.1.103 iodoacetate 1 mM, 97% inhibition Pseudomonas putida
4.2.1.103 KCN 1 mM, 26% inhibition Pseudomonas putida
4.2.1.103 NEM 1 mM,51% inhibition Pseudomonas putida
4.2.1.103 NiSO4 1 mM, 98% inhibition Pseudomonas putida
4.2.1.103 PCMB 1 mM, 50% inhibition Pseudomonas putida
4.2.1.103 phenylmethanesulfonyl fluoride 1 mM, 57% inhibition Pseudomonas putida
4.2.1.103 SnCl2 1 mM, 16% inhibition Pseudomonas putida
4.2.1.103 ZnCl2 1 mM, 34% inhibition Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.103 16.2
-
 cyclohexyl isocyanide
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.103 59000
-
 2 * 59000, SDS-PAGE Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.103 cyclohexyl isocyanide + H2O Pseudomonas putida enzyme is involved in nitrogen-carbon triple bond cleavage N-cyclohexylformamide
-
 ?
4.2.1.103 cyclohexyl isocyanide + H2O Pseudomonas putida N19-2 enzyme is involved in nitrogen-carbon triple bond cleavage N-cyclohexylformamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.103 Pseudomonas putida
-
 N19-2
-
4.2.1.103 Pseudomonas putida N19-2
-
 N19-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.103
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.103 additional information
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.103 benzyl isocyanide 224% of the activity with cyclohexyl isocyanide Pseudomonas putida N-phenylformamide
-
 ?
4.2.1.103 benzyl isocyanide 224% of the activity with cyclohexyl isocyanide Pseudomonas putida N19-2 N-phenylformamide
-
 ?
4.2.1.103 cyclohexyl isocyanide + H2O
-
 Pseudomonas putida N-cyclohexylformamide
-
 ?
4.2.1.103 cyclohexyl isocyanide + H2O enzyme is involved in nitrogen-carbon triple bond cleavage Pseudomonas putida N-cyclohexylformamide
-
 ?
4.2.1.103 cyclohexyl isocyanide + H2O
-
 Pseudomonas putida N19-2 N-cyclohexylformamide
-
 ?
4.2.1.103 cyclohexyl isocyanide + H2O enzyme is involved in nitrogen-carbon triple bond cleavage Pseudomonas putida N19-2 N-cyclohexylformamide
-
 ?
4.2.1.103 isocyanomethyl phosphonic acid diethyl ether + H2O 3% of the activity with cyclohexyl isocyanide Pseudomonas putida formylaminomethyl-phosphonic acid diethyl ester
-
 ?
4.2.1.103 isocyanomethyl phosphonic acid diethyl ether + H2O 3% of the activity with cyclohexyl isocyanide Pseudomonas putida N19-2 formylaminomethyl-phosphonic acid diethyl ester
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.103 dimer 2 * 59000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.103 35
-
-
 Pseudomonas putida

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.103 20 45 20°C: about 55% of maximal activity, 45°C: about 55% of maximal activity Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.103 10
-
 30 min, 10% v/v glycerol, 3% loss of activity Pseudomonas putida
4.2.1.103 20
-
 30 min, 10% v/v glycerol, 11% loss of activity Pseudomonas putida
4.2.1.103 30
-
 30 min, 10% v/v glycerol, 16% loss of activity Pseudomonas putida
4.2.1.103 35
-
 30 min, 10% v/v glycerol, 22% loss of activity Pseudomonas putida
4.2.1.103 40
-
 30 min, 10% v/v glycerol, 31% loss of activity Pseudomonas putida
4.2.1.103 45
-
 30 min, 10% v/v glycerol, 47% loss of activity Pseudomonas putida
4.2.1.103 50
-
 30 min, 10% v/v glycerol, 67% loss of activity Pseudomonas putida
4.2.1.103 55
-
 30 min, 10% v/v glycerol, 81% loss of activity Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.103 6 6.5
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.103 5.5 9 pH 5.5: about 45% of maximal activity, potassium phosphate buffer, pH 9.0: about 30% of maximal activity, NH4OH/NH4Cl buffer Pseudomonas putida