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Literature summary extracted from

  • Kutchan, T.M.; Dittrich, H.
    Characterization and mechanism of the berberine bridge enzyme, a covalently flavinylated oxidase of benzophenanthridine alkaloid biosynthesis in plants (1995), J. Biol. Chem., 270, 24475-24481.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.21.3.3 expressed in Spodoptera frugiperda Sf9 cells Eschscholzia californica

Protein Variants

EC Number Protein Variants Comment Organism
1.21.3.3 H104T no activity Eschscholzia californica
1.21.3.3 H308S 5% activity of wild-type Eschscholzia californica
1.21.3.3 H39G 40% activity of wild-type Eschscholzia californica
1.21.3.3 R100T no activity Eschscholzia californica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.21.3.3 (S)-scoulerine 50% inhibition at 0.01 mM Eschscholzia californica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.3.3 (S)-reticuline + O2 Eschscholzia californica
-
 (S)-scoulerine + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.3.3 Eschscholzia californica
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.3.3 (R,S)-6-O-methyllaudanosoline + O2
-
 Eschscholzia californica ? + H2O2
-
 ?
1.21.3.3 (R,S)-crassifoline + O2
-
 Eschscholzia californica ? + H2O2
-
 ?
1.21.3.3 (R,S)-laudanosoline + O2 specific for the (S)-enantiomer Eschscholzia californica ? + H2O2
-
 ?
1.21.3.3 (S)-N-methylcoclaurine + O2
-
 Eschscholzia californica (S)-coclaurine + H2O2
-
 ?
1.21.3.3 (S)-protosinomenine + O2
-
 Eschscholzia californica ? + H2O2
-
 ?
1.21.3.3 (S)-reticuline + O2
-
 Eschscholzia californica (S)-scoulerine + H2O2
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.21.3.3 10.5
-
-
 Eschscholzia californica

Cofactor

EC Number Cofactor Comment Organism Structure
1.21.3.3 FAD 1 mol per mol protein Eschscholzia californica
1.21.3.3 FAD covalently attached to His104 Eschscholzia californica