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Literature summary extracted from
- Heterologous expression of the plant proteins strictosidine synthase and berberine bridge enzyme in insect cell culture (1994), Phytochemistry, 35, 353-360.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.21.3.3 | expressed in Spodoptera frugiperda Sf9 cells | Eschscholzia californica |
| 4.3.3.2 | expressed in Sf9 cells using the Bacullovirus system, enzyme is secreted into the medium | Rauvolfia serpentina |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.3.3.2 | tryptamine | inhibitory above 0.7 mM | Rauvolfia serpentina |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.21.3.3 | 0.003 | - |
(S)-reticuline | - |
Eschscholzia californica | |
| 4.3.3.2 | 1 | - |
tryptamine | - |
Rauvolfia serpentina | |
| 4.3.3.2 | 1 | - |
secologanin | - |
Rauvolfia serpentina | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.21.3.3 | (S)-reticuline + O2 | Eschscholzia californica | - |
(S)-scoulerine + H2O2 | - |
? | |
| 4.3.3.2 | tryptamine + secologanin | Rauvolfia serpentina | - |
3-alpha(S)-strictosidine | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.21.3.3 | Eschscholzia californica | - |
- |
- |
| 4.3.3.2 | Rauvolfia serpentina | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.21.3.3 | expressed in Sf9 cells | Eschscholzia californica |
| 4.3.3.2 | from serum free medium of transformed Sf9 cells | Rauvolfia serpentina |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.3.3.2 | 3.72 | - |
purified enzyme | Rauvolfia serpentina |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.21.3.3 | (S)-reticuline + O2 | - |
Eschscholzia californica | (S)-scoulerine + H2O2 | - |
? | |
| 4.3.3.2 | tryptamine + secologanin | - |
Rauvolfia serpentina | 3-alpha(S)-strictosidine | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.21.3.3 | 45 | - |
- |
Eschscholzia californica |
| 4.3.3.2 | 55 | - |
- |
Rauvolfia serpentina |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.3.3.2 | 6.5 | - |
- |
Rauvolfia serpentina |
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Release 2023.1 (January 2023)
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