EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.19.67 | Ancymidole | - |
Papaver somniferum | |
1.14.19.67 | CO | in darkness but not in light | Papaver somniferum | |
1.14.19.67 | Prochloraz | - |
Papaver somniferum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.19.67 | 0.017 | - |
(R)-reticuline | - |
Papaver somniferum | |
1.14.19.67 | 0.15 | - |
[reduced NADPH-hemoprotein reductase] | - |
Papaver somniferum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.19.67 | microsome | membrane-bound | Papaver somniferum | - |
- |
1.14.19.67 | mitochondrion | - |
Papaver somniferum | 5739 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.67 | (R)-reticuline + [reduced NADPH-hemoprotein reductase] + O2 | Papaver somniferum | formation of salutaridine, a key intermediate in morphine biosynthesis | salutaridine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.19.67 | Papaver somniferum | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.19.67 | (R)-reticuline + [reduced NADPH-hemoprotein reductase] + O2 = salutaridine + [oxidized NADPH-hemoprotein reductase] + 2 H2O | regioselective and stereoselective para-ortho oxidative coupling | Papaver somniferum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.19.67 | cell suspension culture | thebaine-producing | Papaver somniferum | - |
1.14.19.67 | additional information | no enzyme activity in latex | Papaver somniferum | - |
1.14.19.67 | plant capsule | - |
Papaver somniferum | - |
1.14.19.67 | root | - |
Papaver somniferum | - |
1.14.19.67 | shoot | - |
Papaver somniferum | - |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.19.67 | -20°C, microsome-bound enzyme, freezing causes a total loss of activity | Papaver somniferum |
1.14.19.67 | -70°C, microsome-bound enzyme, 20% loss of activity after 1 month | Papaver somniferum |
1.14.19.67 | 22°C, microsome-bound enzyme, half-life: 2 h | Papaver somniferum |
1.14.19.67 | in ice water, microsome-bound enzyme, half-life: 20 h | Papaver somniferum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.67 | (R)-reticuline + [reduced NADPH-hemoprotein reductase] + O2 | strictly dependent on NADPH as reducing cofactor and on (R)-configurated reticuline | Papaver somniferum | salutaridine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.19.67 | (R)-reticuline + [reduced NADPH-hemoprotein reductase] + O2 | formation of salutaridine, a key intermediate in morphine biosynthesis | Papaver somniferum | salutaridine + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.19.67 | 20 | 25 | - |
Papaver somniferum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.19.67 | 10 | 30 | 10°C: about 30% of maximal activity, 30°C: about 65% of maximal activity | Papaver somniferum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.19.67 | 7.5 | - |
- |
Papaver somniferum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.14.19.67 | 6.5 | 8.5 | about 50% of maximal activity at pH 6.5 and pH 8.5 | Papaver somniferum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.19.67 | cytochrome P450 | enzyme contains cytochrome P450 | Papaver somniferum |