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Literature summary extracted from

  • Colby, J.; Zatman, L.J.
    Purification and properties of the trimethylamine dehydrogenase of bacterium 4B6 (1974), Biochem. J., 143, 555-567.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.8.2 1-phenylethylhydrazine potent inhibition at very low concentration methylotrophic bacterium
1.5.8.2 2-phenylcyclopropylamine 82% inhibition at 0.1 mM methylotrophic bacterium
1.5.8.2 2-phenylethylhydrazine potent inhibition at very low concentration methylotrophic bacterium
1.5.8.2 Ag+ strong inhibition at 1 mM methylotrophic bacterium
1.5.8.2 benzylhydrazine potent inhibition at very low concentration methylotrophic bacterium
1.5.8.2 Betaine hydrochloride 37% inhibition at 1 mM methylotrophic bacterium
1.5.8.2 choline chloride potent inhibition at 1 mM methylotrophic bacterium
1.5.8.2 Co2+ strong inhibition at 1 mM methylotrophic bacterium
1.5.8.2 Cu2+ strong inhibition at 1 mM methylotrophic bacterium
1.5.8.2 Harmaline 65% inhibition at 0.1 mM methylotrophic bacterium
1.5.8.2 hexamethylene bis-trimethylammonium chloride potent inhibition at 1 mM methylotrophic bacterium
1.5.8.2 Hg2+ strong inhibition at 1 mM methylotrophic bacterium
1.5.8.2 iodoacetamide inhibition at 1 mM methylotrophic bacterium
1.5.8.2 Iproniazid potent inhibition at very low concentration methylotrophic bacterium
1.5.8.2 isocarboxazid potent inhibition at very low concentration methylotrophic bacterium
1.5.8.2 isopropylhydrazine potent inhibition at very low concentration methylotrophic bacterium
1.5.8.2 N,N,N-trimethyl-N-phenylammonium chloride potent inhibition at 1 mM methylotrophic bacterium
1.5.8.2 N,N,N-trimethylhydrazonium iodide potent inhibition at 1 mM methylotrophic bacterium
1.5.8.2 N-benzyl-N-methylpropargylamine 27% inhibition at 0.1 mM methylotrophic bacterium
1.5.8.2 N-ethylmaleimide inhibition at 1 mM methylotrophic bacterium
1.5.8.2 Ni2+ strong inhibition at 1 mM methylotrophic bacterium
1.5.8.2 nialamid potent inhibition at very low concentration methylotrophic bacterium
1.5.8.2 p-chloromercuribenzoate inhibition at 1 mM methylotrophic bacterium
1.5.8.2 phenylhydrazine potent inhibition at very low concentration methylotrophic bacterium
1.5.8.2 Tetramethylammonium chloride potent inhibition at 1 mM methylotrophic bacterium
1.5.8.2 trimethylamine inhibitory at higher concentrations methylotrophic bacterium
1.5.8.2 trimethylsulfomium chloride potent inhibition at 1 mM methylotrophic bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.8.2 0.002
-
 trimethylamine electron acceptor: phenazine methosulfate methylotrophic bacterium
1.5.8.2 0.002
-
 ethyldimethylamine electron acceptor: phenazine methosulfate methylotrophic bacterium
1.5.8.2 0.002
-
 2-chloroethyldimethylamine electron acceptor: phenazine methosulfate methylotrophic bacterium
1.5.8.2 0.006
-
 2-hydroxyethyldimethylamine electron acceptor: phenazine methosulfate methylotrophic bacterium
1.5.8.2 0.008
-
 diethylmethylamine electron acceptor: phenazine methosulfate methylotrophic bacterium
1.5.8.2 0.067
-
 2-aminoethyldimethylamine electron acceptor: phenazine methosulfate methylotrophic bacterium
1.5.8.2 0.13
-
 triethylamine electron acceptor: phenazine methosulfate methylotrophic bacterium
1.5.8.2 1.25
-
 phenazine methosulfate substrate: trimethylamine methylotrophic bacterium
1.5.8.2 1.7
-
 Diethylamine electron acceptor: phenazine methosulfate methylotrophic bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.8.2 161000
-
 gel filtration methylotrophic bacterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.8.2 trimethylamine + H2O + electron acceptor methylotrophic bacterium electron acceptors: phenazine methosulfate, brilliant cresyl blue, methylene blue dimethylamine + formaldehyde + reduced electron acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.8.2 methylotrophic bacterium
-
-
-
1.5.8.2 methylotrophic bacterium 4B6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.8.2 to homogeneity, chromatography steps methylotrophic bacterium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.8.2 cell culture
-
 methylotrophic bacterium
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.8.2 2
-
-
 methylotrophic bacterium

Storage Stability

EC Number Storage Stability Organism
1.5.8.2 -20°C, 20 mM sodium phosphate, pH 7.5, 0.4 mg protein/ml, stable for 3 months methylotrophic bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.8.2 2-aminoethyldimethylamine + H2O + phenazine methosulfate 10% of the activity compared to trimethylamine methylotrophic bacterium ? + reduced phenazine methosulfate
-
 ?
1.5.8.2 2-aminoethyldimethylamine + H2O + phenazine methosulfate 10% of the activity compared to trimethylamine methylotrophic bacterium 4B6 ? + reduced phenazine methosulfate
-
 ?
1.5.8.2 2-chloroethyldimethylamine + H2O + phenazine methosulfate 40% of the activity compared to trimethylamine methylotrophic bacterium ? + reduced phenazine methosulfate
-
 ?
1.5.8.2 2-hydroxyethyldimethylamine + H2O + phenazine methosulfate 70% of the activity compared to trimethylamine methylotrophic bacterium ? + reduced phenazine methosulfate
-
 ?
1.5.8.2 diethylamine + H2O + phenazine methosulfate 47% of the activity compared to trimethylamine methylotrophic bacterium ethylamine + acetaldehyde + reduced phenazine methosulfate
-
 ?
1.5.8.2 diethylamine + H2O + phenazine methosulfate 47% of the activity compared to trimethylamine methylotrophic bacterium 4B6 ethylamine + acetaldehyde + reduced phenazine methosulfate
-
 ?
1.5.8.2 diethylmethylamine + H2O + phenazine methosulfate 11% of the activity compared to trimethylamine methylotrophic bacterium diethylamine + ethylmethylamine + formaldehyde + acetaldehyde + reduced phenazine methosulfate
-
 ?
1.5.8.2 diethylmethylamine + H2O + phenazine methosulfate 11% of the activity compared to trimethylamine methylotrophic bacterium 4B6 diethylamine + ethylmethylamine + formaldehyde + acetaldehyde + reduced phenazine methosulfate
-
 ?
1.5.8.2 ethyldimethylamine + H2O + phenazine methosulfate 16% of the activity compared to trimethylamine methylotrophic bacterium ethylmethylamine + formaldehyde + reduced phenazine methosulfate
-
 ?
1.5.8.2 ethyldimethylamine + H2O + phenazine methosulfate 16% of the activity compared to trimethylamine methylotrophic bacterium 4B6 ethylmethylamine + formaldehyde + reduced phenazine methosulfate
-
 ?
1.5.8.2 triethylamine + H2O + phenazine methosulfate 3% of the activity compared to trimethylamine methylotrophic bacterium diethylamine + acetaldehyde + reduced phenazine methosulfate
-
 ?
1.5.8.2 trimethylamine + H2O + electron acceptor electron acceptors: phenazine methosulfate, brilliant cresyl blue, methylene blue methylotrophic bacterium dimethylamine + formaldehyde + reduced electron acceptor
-
 ?
1.5.8.2 trimethylamine + H2O + phenazine methosulfate
-
 methylotrophic bacterium dimethylamine + formaldehyde + reduced phenazine methosulfate
-
 ?
1.5.8.2 trimethylamine + H2O + phenazine methosulfate
-
 methylotrophic bacterium 4B6 dimethylamine + formaldehyde + reduced phenazine methosulfate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.8.2 60
-
 loss of 50% of activity in 20 min methylotrophic bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.8.2 8.5
-
-
 methylotrophic bacterium