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Literature summary extracted from

  • Lampreia, J.; Moura, I.; Teixeira, M.; Peck, H.D.; LeGall, J.; Huynh, B.H.; Moura, J.J.G.
    The active centers of adenylylsulfate reductase from Desulfovibrio gigas. Characterization and spectroscopic studies (1990), Eur. J. Biochem., 188, 653-664.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.99.2 Iron iron sulfur protein, contains 8 iron atoms per 93000 Da protein, two [4Fe-4S] clusters Megalodesulfovibrio gigas

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.99.2 26000
-
 x * 70000 + x * 26000, SDS-PAGE Megalodesulfovibrio gigas
1.8.99.2 70000
-
 x * 70000 + x * 26000, SDS-PAGE Megalodesulfovibrio gigas
1.8.99.2 400000
-
 gel filtration Megalodesulfovibrio gigas

Organism

EC Number Organism UniProt Comment Textmining
1.8.99.2 Megalodesulfovibrio gigas
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.99.2
-
 Megalodesulfovibrio gigas

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.99.2 6
-
-
 Megalodesulfovibrio gigas

Storage Stability

EC Number Storage Stability Organism
1.8.99.2 -20°C, much loss of activity after 30 days Megalodesulfovibrio gigas

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.99.2 adenylyl sulfate + dithionite
-
 Megalodesulfovibrio gigas AMP + sulfite + ?
-
 ?
1.8.99.2 adenylyl sulfate + reduced methyl viologen
-
 Megalodesulfovibrio gigas AMP + sulfite + methyl viologen
-
 ?
1.8.99.2 AMP + sulfite + ferricyanide
-
 Megalodesulfovibrio gigas adenylyl sulfate + ferrocyanide
-
 ?
1.8.99.2 CMP + sulfite + ferricyanide 41% of the activity with AMP Megalodesulfovibrio gigas cytidylyl sulfate + ferrocyanide
-
 ?
1.8.99.2 GMP + sulfite + ferricyanide GMP is as active as AMP Megalodesulfovibrio gigas guanylyl sulfate + ferrocyanide
-
 ?
1.8.99.2 UMP + sulfite + ferricyanide 36% of the activity with AMP Megalodesulfovibrio gigas uridylyl sulfate + ferrocyanide
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.99.2 ? x * 70000 + x * 26000, SDS-PAGE Megalodesulfovibrio gigas

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.99.2 7.4
-
-
 Megalodesulfovibrio gigas

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.99.2 FAD enzyme contains 1 mol of FAD per mol of enzyme Megalodesulfovibrio gigas