Any feedback?
Literature summary extracted from
- The function of the [4Fe-4S] clusters and FAD in bacterial and archaeal adenylylsulfate reductases: evidence for flavin-catalyzed reduction of adenosine 5'-phosphosulfate (2002), J. Biol. Chem., 277, 26066-26073.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.99.2 | AMP | - |
Desulfovibrio vulgaris |  |
| 1.8.99.2 | AMP | above 0.2 mM | Thiobacillus denitrificans | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.99.2 | 0.015 | - |
adenylyl sulfate | - |
Archaeoglobus fulgidus | |
| 1.8.99.2 | 0.02 | - |
AMP | - |
Thiobacillus denitrificans | |
| 1.8.99.2 | 0.05 | - |
AMP | - |
Desulfovibrio vulgaris | |
| 1.8.99.2 | 0.13 | - |
sulfite | - |
Desulfovibrio vulgaris | |
| 1.8.99.2 | 0.17 | - |
AMP | - |
Desulfovibrio desulfuricans | |
| 1.8.99.2 | 0.18 | - |
sulfite | - |
Desulfovibrio desulfuricans | |
| 1.8.99.2 | 0.2 | - |
sulfite | - |
Desulfovibrio vulgaris | |
| 1.8.99.2 | 0.3 | - |
AMP | - |
Desulfovibrio vulgaris | |
| 1.8.99.2 | 0.34 | - |
sulfite | - |
Desulfovibrio vulgaris | |
| 1.8.99.2 | 1.2 | - |
sulfite | - |
Thiobacillus denitrificans | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.99.2 | Iron | - |
Desulfovibrio desulfuricans | |
| 1.8.99.2 | Iron | - |
Thiobacillus denitrificans | |
| 1.8.99.2 | Iron | contains 5.6 iron per alpha,beta heterodimer, novel Fe-S structure, possibly with an iron-nuclearity greater than four | Desulfovibrio vulgaris | |
| 1.8.99.2 | Iron | two [4Fe-4S] clusters, one cluster is deeply buried in the protein matrix, Fe-S center I, center II is located at the surface of the protein | Archaeoglobus fulgidus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.99.2 | 25600 | - |
alpha2beta2, 2 * 67800 + 2 * 25600, smallest catalytic active unit, SDS-PAGE | Desulfovibrio vulgaris |
| 1.8.99.2 | 67800 | - |
alpha2beta2, 2 * 67800 + 2 * 25600, smallest catalytic active unit, SDS-PAGE | Desulfovibrio vulgaris |
| 1.8.99.2 | 186000 | - |
smallest catalytic active subunit, also aggregations of 324000 Da and 569000 Da are detected, gel filtration | Desulfovibrio vulgaris |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.99.2 | adenylyl sulfate + reduced acceptor | Desulfovibrio desulfuricans | key step in sulfur cycle | AMP + sulfite + acceptor | - |
? | |
| 1.8.99.2 | adenylyl sulfate + reduced acceptor | Thiobacillus denitrificans | key step in sulfur cycle | AMP + sulfite + acceptor | - |
? | |
| 1.8.99.2 | adenylyl sulfate + reduced acceptor | Desulfovibrio vulgaris | key step in sulfur cycle | AMP + sulfite + acceptor | - |
? | |
| 1.8.99.2 | adenylyl sulfate + reduced acceptor | Archaeoglobus fulgidus | key step in sulfur cycle | AMP + sulfite + acceptor | - |
? | |
| 1.8.99.2 | adenylyl sulfate + reduced acceptor | Desulfovibrio vulgaris Hildenborough | key step in sulfur cycle | AMP + sulfite + acceptor | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.99.2 | Archaeoglobus fulgidus | - |
- |
- |
| 1.8.99.2 | Desulfovibrio desulfuricans | - |
- |
- |
| 1.8.99.2 | Desulfovibrio vulgaris | - |
- |
- |
| 1.8.99.2 | Desulfovibrio vulgaris Hildenborough | - |
- |
- |
| 1.8.99.2 | Thiobacillus denitrificans | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.99.2 | - |
Desulfovibrio desulfuricans |
| 1.8.99.2 | - |
Desulfovibrio vulgaris |
| 1.8.99.2 | - |
Archaeoglobus fulgidus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.8.99.2 | AMP + sulfite + acceptor = adenylyl sulfate + reduced acceptor | mechanism | Desulfovibrio desulfuricans | |
| 1.8.99.2 | AMP + sulfite + acceptor = adenylyl sulfate + reduced acceptor | mechanism | Thiobacillus denitrificans | |
| 1.8.99.2 | AMP + sulfite + acceptor = adenylyl sulfate + reduced acceptor | mechanism | Desulfovibrio vulgaris | |
| 1.8.99.2 | AMP + sulfite + acceptor = adenylyl sulfate + reduced acceptor | mechanism | Archaeoglobus fulgidus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.99.2 | adenylyl sulfate + dithionite | - |
Archaeoglobus fulgidus | AMP + sulfite + ? | - |
? | |
| 1.8.99.2 | adenylyl sulfate + reduced acceptor | key step in sulfur cycle | Desulfovibrio desulfuricans | AMP + sulfite + acceptor | - |
? | |
| 1.8.99.2 | adenylyl sulfate + reduced acceptor | key step in sulfur cycle | Thiobacillus denitrificans | AMP + sulfite + acceptor | - |
? | |
| 1.8.99.2 | adenylyl sulfate + reduced acceptor | key step in sulfur cycle | Desulfovibrio vulgaris | AMP + sulfite + acceptor | - |
? | |
| 1.8.99.2 | adenylyl sulfate + reduced acceptor | key step in sulfur cycle | Archaeoglobus fulgidus | AMP + sulfite + acceptor | - |
? | |
| 1.8.99.2 | adenylyl sulfate + reduced acceptor | key step in sulfur cycle | Desulfovibrio vulgaris Hildenborough | AMP + sulfite + acceptor | - |
? | |
| 1.8.99.2 | adenylyl sulfate + reduced methyl viologen | - |
Desulfovibrio desulfuricans | AMP + sulfite + methyl viologen | - |
? | |
| 1.8.99.2 | adenylyl sulfate + reduced methyl viologen | - |
Thiobacillus denitrificans | AMP + sulfite + methyl viologen | - |
? | |
| 1.8.99.2 | adenylyl sulfate + reduced methyl viologen | - |
Desulfovibrio vulgaris | AMP + sulfite + methyl viologen | - |
? | |
| 1.8.99.2 | adenylyl sulfate + reduced methyl viologen | - |
Archaeoglobus fulgidus | AMP + sulfite + methyl viologen | - |
? | |
| 1.8.99.2 | adenylyl sulfate + reduced methyl viologen | - |
Desulfovibrio vulgaris Hildenborough | AMP + sulfite + methyl viologen | - |
? | |
| 1.8.99.2 | AMP + sulfite + ferricyanide | - |
Desulfovibrio desulfuricans | adenylyl sulfate + ferrocyanide | - |
? | |
| 1.8.99.2 | AMP + sulfite + ferricyanide | - |
Thiobacillus denitrificans | adenylyl sulfate + ferrocyanide | - |
? | |
| 1.8.99.2 | AMP + sulfite + ferricyanide | - |
Desulfovibrio vulgaris | adenylyl sulfate + ferrocyanide | - |
? | |
| 1.8.99.2 | AMP + sulfite + ferricyanide | - |
Archaeoglobus fulgidus | adenylyl sulfate + ferrocyanide | - |
? | |
| 1.8.99.2 | AMP + sulfite + ferricyanide | - |
Desulfovibrio vulgaris Hildenborough | adenylyl sulfate + ferrocyanide | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.99.2 | tetramer | - |
Archaeoglobus fulgidus |
| 1.8.99.2 | tetramer | alpha2beta2, 2 * 67800 + 2 * 25600, smallest catalytic active unit, SDS-PAGE | Desulfovibrio vulgaris |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.99.2 | FAD | - |
Desulfovibrio desulfuricans | |
| 1.8.99.2 | FAD | - |
Thiobacillus denitrificans | |
| 1.8.99.2 | FAD | - |
Desulfovibrio vulgaris | |
| 1.8.99.2 | FAD | located nearby Fe-S center I, pointing ist C-8 methyl group to the iron-sulfur center | Archaeoglobus fulgidus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
