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Literature summary extracted from

  • Kopriva, S.; Buchert, T.; Fritz, G.; Suter, M.; Benda, R.; Schunemann, V.; Koprivova, A.; Schurmann, P.; Trautwein, A.X.; Kroneck, P.M.H.; Brunold, C.
    The presence of an iron-sulfur cluster in adenosine 5'-phosphosulfate reductase separates organisms utilizing adenosine 5'-phosphosulfate and phosphoadenosine 5'-phosphosulfate for sulfate assimilation (2002), J. Biol. Chem., 277, 21786-21791.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.8 DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression in strain BL21(DE3) as His-tagged protein Escherichia coli
1.8.99.2
-
 Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.4.8 additional information enzyme contains no iron Escherichia coli
1.8.99.2 Iron contains a [4Fe-4S] cluster Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.8 3'-phosphoadenylyl-sulfate + thioredoxin Escherichia coli
-
 adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
 ?
1.8.4.8 additional information Escherichia coli enzyme is essential for sulfate assimilation using phosphoadenosine 5'-phosphosulfate, not adenosine 5'-phosphosulfate, phylogenetic comparison of the 2 different groups, overview ?
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor Pseudomonas aeruginosa assimilatory sulfate reduction AMP + sulfite + acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.8 Escherichia coli
-
-
-
1.8.99.2 Pseudomonas aeruginosa
-
-
-
1.8.99.2 Sinorhizobium meliloti
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.8 recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.8 3'-phosphoadenylyl-sulfate + thioredoxin
-
 Escherichia coli adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
 ?
1.8.4.8 3'-phosphoadenylyl-sulfate + thioredoxin thioredoxin m from spinach Escherichia coli adenosine 3',5'-bisphosphate + thioredoxin disulfide + sulfite
-
 ?
1.8.4.8 additional information enzyme is essential for sulfate assimilation using phosphoadenosine 5'-phosphosulfate, not adenosine 5'-phosphosulfate, phylogenetic comparison of the 2 different groups, overview Escherichia coli ?
-
 ?
1.8.99.2 adenylyl sulfate + dithioerythritol
-
 Pseudomonas aeruginosa AMP + sulfite + ?
-
 ?
1.8.99.2 adenylyl sulfate + reduced acceptor assimilatory sulfate reduction Pseudomonas aeruginosa AMP + sulfite + acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.4.8 3'-Phosphoadenylylsulfate reductase
-
 Escherichia coli
1.8.4.8 PAPS reductase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.8 thioredoxin
-
 Escherichia coli