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Literature summary extracted from

  • Quentmeier, A.; Kraft, R.; Kostka, S.; Klockenkamper, R.; Friedrich, C.G.
    Characterization of a new type of sulfite dehydrogenase from Paracoccus pantotrophus GB17 (2000), Arch. Microbiol., 173, 117-125.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.2.1 Ni2+ 1 mM, 32% inhibition Paracoccus pantotrophus
1.8.2.1 phosphate
-
 Paracoccus pantotrophus
1.8.2.1 SO42-
-
 Paracoccus pantotrophus
1.8.2.1 Zn2+ 1 mM, 27% inhibition Paracoccus pantotrophus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.2.1 0.007
-
 sulfite
-
 Paracoccus pantotrophus
1.8.2.1 0.019
-
 ferricytochrome c
-
 Paracoccus pantotrophus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.2.1 periplasm
-
 Paracoccus pantotrophus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.2.1 Iron enzyme contains 3.17 mol of iron per mol of enzyme Paracoccus pantotrophus
1.8.2.1 Mg2+ 1 mM, 150% increase in activity Paracoccus pantotrophus
1.8.2.1 Mn2+ 1 mM, 127% increase in activity Paracoccus pantotrophus
1.8.2.1 Molybdenum the 43897 Da subunit contains the 455 Da molybdenum cofactor, 1.3 mol of molybdenum per mol of enzyme Paracoccus pantotrophus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.2.1 38815
-
 2 * 43897 + 2 * 38815, the 43897 Da subunit contains the 455 Da molybdenum cofactor, the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, electrospray ionization mass spectrometry Paracoccus pantotrophus
1.8.2.1 43897
-
 2 * 43897 + 2 * 38815, the 43897 Da subunit contains the 455 Da molybdenum cofactor, the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, electrospray ionization mass spectrometry Paracoccus pantotrophus
1.8.2.1 47000
-
 alpha2beta2, 2 * 47000 + 2 * 50000, SDS-PAGE Paracoccus pantotrophus
1.8.2.1 50000
-
 alpha2beta2, 2 * 47000 + 2 * 50000, SDS-PAGE Paracoccus pantotrophus
1.8.2.1 190000
-
 native gradient PAGE Paracoccus pantotrophus

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.1 Paracoccus pantotrophus
-
 GB17
-
1.8.2.1 Paracoccus pantotrophus GB17
-
 GB17
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.2.1
-
 Paracoccus pantotrophus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.2.1 0.049
-
-
 Paracoccus pantotrophus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.1 sulfite + ferricytochrome c + H2O
-
 Paracoccus pantotrophus sulfate + ferrocytochrome c + H+
-
 ?
1.8.2.1 sulfite + ferricytochrome c + H2O
-
 Paracoccus pantotrophus GB17 sulfate + ferrocytochrome c + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.2.1 tetramer 2 * 43897 + 2 * 38815, the 43897 Da subunit contains the 455 Da molybdenum cofactor, the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, electrospray ionization mass spectrometry Paracoccus pantotrophus
1.8.2.1 tetramer alpha2beta2, 2 * 47000 + 2 * 50000, SDS-PAGE Paracoccus pantotrophus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.2.1 6.5
-
-
 Paracoccus pantotrophus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.2.1 heme the 38815 Da subunit consists of a polypeptide and two covalently bound hemes, 3.53 mol of heme per mol of enzyme Paracoccus pantotrophus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.2.1 45
-
 phosphate
-
 Paracoccus pantotrophus