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Literature summary extracted from

  • Kappler, U.; Bennett, B.; Rethmeier, J.; Schwarz, G.; Deutzmann, R.; McEwan, A.G.; Dahl, C.
    Sulfite:cytochrome c oxidoreductase from Thiobacillus novellus. Purification, characterization, and molecular biology of a heterodimeric member of the sulfite oxidase family (2000), J. Biol. Chem., 275, 13202-13212.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.2.1
-
Starkeya novella

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.2.1 SO42- product inhibition, mixed-type non-competitive with respect to sulfite Starkeya novella

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.2.1 0.0025
-
ferricytochrome c550
-
Starkeya novella
1.8.2.1 0.004
-
ferricytochrome c
-
Starkeya novella
1.8.2.1 0.027
-
sulfite
-
Starkeya novella

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.2.1 periplasm
-
Starkeya novella
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.2.1 Molybdenum contains 1.2 Mo atoms per holoenzyme Starkeya novella

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.2.1 46000
-
gel filtration Starkeya novella

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.1 Starkeya novella
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.2.1
-
Starkeya novella

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.2.1 sulfite + 2 ferricytochrome c + H2O = sulfate + 2 ferrocytochrome c + 2 H+ ping-pong mechanism Starkeya novella

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.2.1 327.9
-
-
Starkeya novella

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.1 sulfite + ferricytochrome c + H2O
-
Starkeya novella sulfate + ferrocytochrome c + H+
-
?
1.8.2.1 sulfite + ferricytochrome c550 + H2O
-
Starkeya novella sulfate + ferrocytochrome c550 + H+
-
r

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.2.1 cytochrome c550 enzyme reacts exclusively with cytochrome c550 Starkeya novella
1.8.2.1 heme the enzyme contains 0.8 heme groups per holoenzyme Starkeya novella

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.2.1 additional information
-
additional information
-
Starkeya novella