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Literature summary extracted from

  • Lyric, R.M.; Suzuki, I.
    Enzymes involved in the metabolism of thiosulfate by Thiobacillus thioparus. 3. Properties of thiosulfate-oxidizing enzyme and proposed pathway of thiosulfate oxidation (1970), Can. J. Biochem., 48, 355-363.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.2.2 2,2'-bipyridyl 50% inhibition at 1 mM, a 60 min incubation period is necessary to potentiate the inhibition Thiobacillus thioparus
1.8.2.2 EDTA 44% inhibition at 10 mM, a 15 min incubation period is necessary to potentiate the inhibition Thiobacillus thioparus
1.8.2.2 HgCl2 100% inhibition at 0.5 mM Thiobacillus thioparus
1.8.2.2 N-ethylmaleimide 100% inhibition at 1 mM, a 15 min incubation period is necessary to potentiate the inhibition Thiobacillus thioparus
1.8.2.2 p-chloromercuribenzoate 72.8% inhibition at 1 mM Thiobacillus thioparus
1.8.2.2 SO32- 50% inhibition at 0.005 mM , time-dependent inhibition, irreversible Thiobacillus thioparus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.2.2 additional information
-
 additional information
-
 Thiobacillus thioparus
1.8.2.2 0.005
-
 thiosulfate cytochrome c as electron acceptor Thiobacillus thioparus
1.8.2.2 0.1
-
 thiosulfate ferricyanide as electron acceptor Thiobacillus thioparus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.2.2 Iron enzyme contains 2 mol of non-heme iron Thiobacillus thioparus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.2.2 115000
-
 sucrose density gradient method Thiobacillus thioparus

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.2 Thiobacillus thioparus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.2.2 using sonication, column chromatography on DEAE-cellulose, pH-adjustment to 4.5 and calcium phosphate gel treatment Thiobacillus thioparus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.2.2 146.1
-
-
 Thiobacillus thioparus

Storage Stability

EC Number Storage Stability Organism
1.8.2.2 -20°C, the ability of the enzyme to couple with cytochrome c decreases during storage Thiobacillus thioparus
1.8.2.2 4°C, 10% loss of activity after 6 h and 50% loss of activity after 5 days Thiobacillus thioparus
1.8.2.2 frozen, stable, indefinitely as measured by ferricyanide reduction Thiobacillus thioparus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.2 additional information NAD+, methylene blue, 2,6-dichlorophenol indophenol Thiobacillus thioparus ?
-
 ?
1.8.2.2 additional information no activity with: NADP+, O2 Thiobacillus thioparus ?
-
 ?
1.8.2.2 thiosulfate + oxidized acceptor cytochrome c of yeast is a poor electron acceptor Thiobacillus thioparus tetrathionate + reduced acceptor
-
 ?
1.8.2.2 thiosulfate + oxidized acceptor horse cytochrome c is a poor electron acceptor Thiobacillus thioparus tetrathionate + reduced acceptor
-
 ?
1.8.2.2 thiosulfate + oxidized acceptor cytochrome c acts as electron acceptor Thiobacillus thioparus tetrathionate + reduced acceptor
-
 ?
1.8.2.2 thiosulfate + oxidized acceptor ferricyanide is a much better electron acceptor than cytochrome c Thiobacillus thioparus tetrathionate + reduced acceptor
-
 ?
1.8.2.2 thiosulfate + oxidized acceptor ferricyanide acts as electron acceptor Thiobacillus thioparus tetrathionate + reduced acceptor
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.2.2 4
-
 6 h, 10% loss of activity, 50% loss of activity after 5 days Thiobacillus thioparus
1.8.2.2 55
-
 5 min, stable Thiobacillus thioparus
1.8.2.2 60
-
 5 min, 50% loss of activity Thiobacillus thioparus
1.8.2.2 100
-
 1 min, complete loss of activity Thiobacillus thioparus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.2.2 5
-
 below, using ferricyanide as electron acceptor Thiobacillus thioparus
1.8.2.2 6.5
-
 broad, with cytochrome c as electron acceptor Thiobacillus thioparus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.2.2 5.5 8.8 little change in activity between Thiobacillus thioparus