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Literature summary extracted from

  • Lostanlen, D.; De Barro, A.V.; Leroux, A.; Kaplan, J.C.
    Soluble NADH-cytochrome b5 reductase from rabbit liver cytosol: partial purification and characterization (1978), Biochim. Biophys. Acta, 526, 42-51.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.2 p-hydroxymercuribenzoate 0.1 mM, complete inhibition Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 0.008
-
 methemoglobin-ferrocyanide complex
-
 Oryctolagus cuniculus
1.6.2.2 0.01
-
 ferricyanide
-
 Oryctolagus cuniculus
1.6.2.2 0.015
-
 cytochrome b5
-
 Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.2 cytosol
-
 Oryctolagus cuniculus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.2 25000
-
 liver, cytosol, gel filtration, sucrose density gradient centrifugation Oryctolagus cuniculus
1.6.2.2 30000
-
 enzyme from liver cytosol, gel filtration Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.2
-
 Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.2 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.2 1.69
-
 methemoglobin-ferrocyanide reductases assay Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
1.6.2.2 -20°C, several months, no loss of activity Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 2 ferricytochrome b5 + NADH specific for NADH as electron donor, artificial acceptors: ferricyanide, 2,6-dichlorphenolindophenol Oryctolagus cuniculus 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 2 ferricytochrome b5 + NADH additional acceptor: methemoglobin-ferrocyanide complex Oryctolagus cuniculus 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 methemoglobin-ferrocyanide complex + NADH
-
 Oryctolagus cuniculus reduced methemoglobin-ferrocyanide complex + NAD+
-
 ?
1.6.2.2 NADH + ferricyanide
-
 Oryctolagus cuniculus NAD+ + H+ + ferrocyanide
-
 r
1.6.2.2 NADH + ferricytochrome b5
-
 Oryctolagus cuniculus NAD+ + H+ + ferrocytochrome b5
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.2 6.5
-
 Tris-phosphate buffer Oryctolagus cuniculus
1.6.2.2 7
-
 triethanolamine buffer Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.2 NADH
-
 Oryctolagus cuniculus