EC Number | Cloned (Comment) | Organism |
---|---|---|
1.6.2.2 | expression of wild-type, H49A, H49E, H49K, H49Y, and DELTAF272 mutant enzymes in Escherichia coli | Sus scrofa |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.6.2.2 | H49A | reduced kcat | Sus scrofa |
1.6.2.2 | H49E | elevated Km value for cytochrome b5, strongly reduced kcat | Sus scrofa |
1.6.2.2 | H49K | reduced Km value for cytochrome b5 | Sus scrofa |
1.6.2.2 | H49Y | similar to wild-type | Sus scrofa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.6.2.2 | 0.0003 | - |
NADH | H49E mutant enzyme | Sus scrofa | |
1.6.2.2 | 0.0006 | - |
ferricyanide | H49K mutant enzyme | Sus scrofa | |
1.6.2.2 | 0.00064 | - |
NADH | G273 mutant enzyme | Sus scrofa | |
1.6.2.2 | 0.00084 | - |
NADH | DELTAF272 mutant enzyme | Sus scrofa | |
1.6.2.2 | 0.0015 | - |
NADH | recombinant wild-type enzyme | Sus scrofa | |
1.6.2.2 | 0.0016 | - |
NADH | native enzyme | Sus scrofa | |
1.6.2.2 | 0.0019 | - |
NADH | H49A mutant enzyme | Sus scrofa | |
1.6.2.2 | 0.0022 | - |
ferricyanide | H49E mutant enzyme | Sus scrofa | |
1.6.2.2 | 0.0025 | - |
ferricyanide | native enzyme | Sus scrofa | |
1.6.2.2 | 0.0025 | - |
cytochrome b5 | G273 mutant enzyme | Sus scrofa | |
1.6.2.2 | 0.0026 | - |
ferricyanide | H49Y mutant enzyme | Sus scrofa | |
1.6.2.2 | 0.0027 | - |
NADH | H49Y mutant enzyme | Sus scrofa | |
1.6.2.2 | 0.0028 | - |
ferricyanide | recombinant wild-type enzyme | Sus scrofa | |
1.6.2.2 | 0.0028 | - |
ferricyanide | H49A mutant enzyme | Sus scrofa | |
1.6.2.2 | 0.0031 | - |
cytochrome b5 | H49K mutant enzyme | Sus scrofa | |
1.6.2.2 | 0.0052 | - |
ferricyanide | DELTAF272 mutant enzyme | Sus scrofa | |
1.6.2.2 | 0.0052 | - |
ferricyanide | G273 mutant enzyme | Sus scrofa | |
1.6.2.2 | 0.0088 | - |
cytochrome b5 | native enzyme | Sus scrofa | |
1.6.2.2 | 0.0089 | - |
cytochrome b5 | H49A mutant enzyme | Sus scrofa | |
1.6.2.2 | 0.0091 | - |
cytochrome b5 | recombinant wild-type enzyme | Sus scrofa | |
1.6.2.2 | 0.01 | - |
NADH | H49K mutant enzyme | Sus scrofa | |
1.6.2.2 | 0.011 | - |
cytochrome b5 | H49Y mutant enzyme | Sus scrofa | |
1.6.2.2 | 0.017 | - |
cytochrome b5 | DELTAF272 mutant enzyme | Sus scrofa | |
1.6.2.2 | 0.03 | - |
cytochrome b5 | H49E mutant enzyme | Sus scrofa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.6.2.2 | Sus scrofa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.6.2.2 | recombinant wild-type, H49A, H49E, H49K, H49Y, and DELTAF272 mutant enzyme | Sus scrofa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.6.2.2 | NADH + ferricytochrome b5 | - |
Sus scrofa | NAD+ + H+ + ferrocytochrome b5 | - |
r |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.6.2.2 | 53 | - |
NADH | G273 mutant enzyme | Sus scrofa | |
1.6.2.2 | 57 | - |
ferricytochrome b5 | G273 mutant enzyme | Sus scrofa | |
1.6.2.2 | 76 | - |
ferricyanide | G273 mutant enzyme | Sus scrofa | |
1.6.2.2 | 77 | - |
NADH | H49E mutant enzyme | Sus scrofa | |
1.6.2.2 | 110 | - |
ferricyanide | H49E mutant enzyme | Sus scrofa | |
1.6.2.2 | 160 | - |
ferricytochrome b5 | H49E mutant enzyme | Sus scrofa | |
1.6.2.2 | 310 | - |
NADH | DELTAF272 mutant enzyme | Sus scrofa | |
1.6.2.2 | 380 | - |
ferricytochrome b5 | H49A mutant enzyme | Sus scrofa | |
1.6.2.2 | 390 | - |
NADH | native enzyme | Sus scrofa | |
1.6.2.2 | 400 | - |
ferricyanide | DELTAF272 mutant enzyme | Sus scrofa | |
1.6.2.2 | 420 | - |
ferricytochrome b5 | H49K mutant enzyme | Sus scrofa | |
1.6.2.2 | 440 | - |
NADH | H49A mutant enzyme | Sus scrofa | |
1.6.2.2 | 450 | - |
ferricytochrome b5 | DELTAF272 mutant enzyme | Sus scrofa | |
1.6.2.2 | 470 | - |
ferricyanide | H49A mutant enzyme | Sus scrofa | |
1.6.2.2 | 490 | - |
ferricyanide | H49K mutant enzyme | Sus scrofa | |
1.6.2.2 | 510 | - |
ferricytochrome b5 | native enzyme | Sus scrofa | |
1.6.2.2 | 560 | - |
ferricytochrome b5 | recombinant wild-type enzyme | Sus scrofa | |
1.6.2.2 | 570 | - |
NADH | recombinant wild-type enzyme | Sus scrofa | |
1.6.2.2 | 580 | - |
ferricytochrome b5 | H49Y mutant enzyme | Sus scrofa | |
1.6.2.2 | 630 | - |
ferricyanide | native enzyme | Sus scrofa | |
1.6.2.2 | 710 | - |
NADH | H49K mutant enzyme | Sus scrofa | |
1.6.2.2 | 727 | - |
ferricyanide | H49Y mutant enzyme | Sus scrofa | |
1.6.2.2 | 750 | - |
NADH | H49Y mutant enzyme | Sus scrofa | |
1.6.2.2 | 810 | - |
ferricyanide | recombinant wild-type enzyme | Sus scrofa |