Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kimura, S.; Nishida, H.; Iyanagi, T.
    Effects of flavin-binding motif amino acid mutations in the NADH-cytochrome b5 reductase catalytic domain on protein stability and catalysis (2001), J. Biochem., 130, 481-490.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.2.2 expression of wild-type, K97A, K97R, S99A, S99T, S99V, R63A, R63Q, R63K, Y65A and Y65F mutant enzymes in Escherichia coli Sus scrofa

Protein Variants

EC Number Protein Variants Comment Organism
1.6.2.2 K97A mutation in flavin-binding motif Sus scrofa
1.6.2.2 K97R mutation in flavin-binding motif Sus scrofa
1.6.2.2 R63A mutation in flavin-binding motif Sus scrofa
1.6.2.2 R63K mutation in flavin-binding motif Sus scrofa
1.6.2.2 R63Q mutation in flavin-binding motif Sus scrofa
1.6.2.2 S99A mutation in flavin-binding motif Sus scrofa
1.6.2.2 S99T mutation in flavin-binding motif Sus scrofa
1.6.2.2 S99V mutation in flavin-binding motif Sus scrofa
1.6.2.2 Y65A mutation in flavin-binding motif Sus scrofa
1.6.2.2 Y65F mutation in flavin-binding motif Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 0.00148
-
 ferricytochrome b5 S99A mutant enzyme Sus scrofa
1.6.2.2 0.0019
-
 NADH S99T mutant enzyme Sus scrofa
1.6.2.2 0.0021
-
 NADH R63K mutant enzyme Sus scrofa
1.6.2.2 0.0029
-
 NADH K97R mutant enzyme Sus scrofa
1.6.2.2 0.003
-
 NADH Y65F mutant enzyme Sus scrofa
1.6.2.2 0.0031
-
 NADH recombinant wild-type enzyme Sus scrofa
1.6.2.2 0.0042
-
 NADH Y65A mutant enzyme Sus scrofa
1.6.2.2 0.0044
-
 NADH K97A mutant enzyme Sus scrofa
1.6.2.2 0.005
-
 ferricytochrome b5 Y65A mutant enzyme Sus scrofa
1.6.2.2 0.0062
-
 ferricytochrome b5 Y65F mutant enzyme Sus scrofa
1.6.2.2 0.0069
-
 ferricytochrome b5 K97R mutant enzyme Sus scrofa
1.6.2.2 0.0073
-
 ferricytochrome b5 R63K mutant enzyme Sus scrofa
1.6.2.2 0.0094
-
 NADH S99V mutant enzyme Sus scrofa
1.6.2.2 0.0099
-
 ferricytochrome b5 S99V mutant enzyme Sus scrofa
1.6.2.2 0.0104
-
 ferricytochrome b5 recombinant wild-type enzyme Sus scrofa
1.6.2.2 0.0118
-
 ferricytochrome b5 S99T mutant enzyme Sus scrofa
1.6.2.2 0.0161
-
 ferricytochrome b5 K97A mutant enzyme Sus scrofa
1.6.2.2 0.0185
-
 ferricytochrome b5 R63Q mutant enzyme Sus scrofa
1.6.2.2 0.021
-
 NADH R63Q mutant enzyme Sus scrofa
1.6.2.2 0.026
-
 NADH S99A mutant enzyme Sus scrofa
1.6.2.2 0.0421
-
 ferricytochrome b5 R63A mutant enzyme Sus scrofa
1.6.2.2 0.104
-
 NADH R63A mutant enzyme Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.2 recombinant wild-type, K97A, K97R, S99A, S99T, S99V, R63A, R63Q, R63K, Y65A and Y65F mutant enzymes Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 NADH + ferricytochrome b5
-
 Sus scrofa NAD+ + H+ + ferrocytochrome b5
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.2.2 105
-
 ferricytochrome b5 R63Q mutant enzyme Sus scrofa
1.6.2.2 107
-
 ferricytochrome b5 Y65A mutant enzyme Sus scrofa
1.6.2.2 121
-
 ferricytochrome b5 R63A mutant enzyme Sus scrofa
1.6.2.2 165
-
 ferricytochrome b5 S99V mutant enzyme Sus scrofa
1.6.2.2 180
-
 NADH Y65A mutant enzyme Sus scrofa
1.6.2.2 191
-
 ferricytochrome b5 K97A mutant enzyme Sus scrofa
1.6.2.2 295
-
 ferricytochrome b5 S99A mutant enzyme Sus scrofa
1.6.2.2 305
-
 ferricytochrome b5 Y65F mutant enzyme Sus scrofa
1.6.2.2 347
-
 ferricytochrome b5 R63K mutant enzyme Sus scrofa
1.6.2.2 375
-
 NADH S99V mutant enzyme Sus scrofa
1.6.2.2 403
-
 NADH R63A mutant enzyme Sus scrofa
1.6.2.2 412
-
 NADH R63Q mutant enzyme Sus scrofa
1.6.2.2 415
-
 ferricytochrome b5 K97R mutant enzyme Sus scrofa
1.6.2.2 435
-
 NADH S99A mutant enzyme Sus scrofa
1.6.2.2 501
-
 NADH Y65F mutant enzyme Sus scrofa
1.6.2.2 515
-
 NADH R63K mutant enzyme Sus scrofa
1.6.2.2 520
-
 ferricytochrome b5 S99T mutant enzyme Sus scrofa
1.6.2.2 661
-
 ferricytochrome b5 recombinant wild-type enzyme Sus scrofa
1.6.2.2 851
-
 NADH S99T mutant enzyme Sus scrofa
1.6.2.2 909
-
 NADH K97R mutant enzyme Sus scrofa
1.6.2.2 1060
-
 NADH K97A mutant enzyme Sus scrofa
1.6.2.2 1100
-
 NADH recombinant wild-type enzyme Sus scrofa