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Literature summary extracted from

  • Viljoen, C.C.; Cloete, F.; Botes, D.P.; Kruger, H.
    Isolation and characterization of NAD(P)H-dehydrogenases from seeds of the castor bean (1983), Phytochemistry, 22, 365-370.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.5.2 2,4-dinitrophenol
-
 Ricinus communis
1.6.5.2 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Ricinus communis
1.6.5.2 DTT
-
 Ricinus communis
1.6.5.2 EDTA
-
 Ricinus communis
1.6.5.2 iodoacetic acid
-
 Ricinus communis
1.6.5.2 Thenoyltrifluoroacetone
-
 Ricinus communis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.2 60260
-
 enzyme DII, gel filtration, SDS-PAGE, amino acid analysis Ricinus communis
1.6.5.2 63000
-
 1 * 63000, enzyme DII, SDS-PAGE Ricinus communis
1.6.5.2 66070
-
 enzyme DI, gel filtration, SDS-PAGE, amino acid analysis Ricinus communis
1.6.5.2 67000
-
 1 * 67000, enzyme DI, SDS-PAGE Ricinus communis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.5.2 quinone + NAD(P)H Ricinus communis possible role in seed germination hydroquinone + NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.2 Ricinus communis
-
 castor bean, enzymes DI and DII
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.2 2 isoenzymes: D-I, D-II Ricinus communis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.5.2 seed
-
 Ricinus communis
-

Storage Stability

EC Number Storage Stability Organism
1.6.5.2 5°C, 0.05 M sodium phosphate, pH 8, enzyme D-II is stable for 1 month, enzyme D-I loses 30% of its activity after 1 week Ricinus communis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.2 quinone + NAD(P)H possible role in seed germination Ricinus communis hydroquinone + NAD(P)+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.5.2 monomer 1 * 67000, enzyme DI, SDS-PAGE Ricinus communis
1.6.5.2 monomer 1 * 63000, enzyme DII, SDS-PAGE Ricinus communis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.5.2 7 8.5
-
 Ricinus communis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.6.5.2 5 9.5
-
 Ricinus communis

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.2 NADH
-
 Ricinus communis
1.6.5.2 NADPH
-
 Ricinus communis