Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sengupta, S.; Shaila, M.S.; Rao, G.R.
    Purification and characterization of assimilatory nitrite reductase from Candida utilis (1996), Biochem. J., 317, 147-155.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.7.1 0.069
-
 NADPH
-
 Cyberlindnera jadinii
1.7.7.1 0.08
-
 methyl viologen
-
 Cyberlindnera jadinii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.7.1 58000
-
 1 * 58000 + 1 * 66000, SDS-PAGE Cyberlindnera jadinii
1.7.7.1 66000
-
 1 * 58000 + 1 * 66000, SDS-PAGE Cyberlindnera jadinii
1.7.7.1 126000
-
 gel filtration, native enzyme Cyberlindnera jadinii

Organism

EC Number Organism UniProt Comment Textmining
1.7.7.1 Cyberlindnera jadinii
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.7.7.1 phosphoprotein 66 kDa subunit is phosphorylated Cyberlindnera jadinii

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.7.1 homogeneity Cyberlindnera jadinii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.7.1 16.06
-
-
 Cyberlindnera jadinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.7.1 nitrite + NADPH + H+
-
 Cyberlindnera jadinii NH3 + NADP+ + H2O
-
 r
1.7.7.1 nitrite + reduced ferredoxin NADPH Cyberlindnera jadinii ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced ferredoxin other electron donor: methyl viologen Cyberlindnera jadinii ammonia + oxidized ferredoxin
-
 ?
1.7.7.1 nitrite + reduced methyl viologen
-
 Cyberlindnera jadinii NH3 + H2O + oxidized methyl viologen
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.7.1 dimer 1 * 58000 + 1 * 66000, SDS-PAGE Cyberlindnera jadinii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.7.1 30
-
-
 Cyberlindnera jadinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.7.1 7.5
-
 phosphate buffer Cyberlindnera jadinii
1.7.7.1 8
-
 Tris buffer Cyberlindnera jadinii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.7.7.1 7.8 8.3 appreciable amounts of activity at pH 7.8 and pH 8.3 in Tris buffer Cyberlindnera jadinii