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Literature summary extracted from

  • Bernheim, M.L.C.
    The hydroxylamine reductase of mitochondria (1969), Arch. Biochem. Biophys., 134, 408-413.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.7.1.10 dithiothreitol stabilizes during storage Rattus norvegicus
1.7.1.10 freezing partially inactivates membrane bound enzyme Rattus norvegicus
1.7.1.10 NADH prevents heat damage Rattus norvegicus
1.7.1.10 solubilization with deoxycholate, n-butanol or acetone destroys membrane bound enzyme Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.10 HgCl2
-
 Rattus norvegicus
1.7.1.10 N-ethylmaleimide not inhibited Rattus norvegicus
1.7.1.10 p-chloromercuribenzoate 0.05 mM, complete inhibition, subsequent addition of 3 mM cysteine restores the activity Rattus norvegicus
1.7.1.10 phosphate
-
 Rattus norvegicus
1.7.1.10 Sodium arsenate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.10 0.5
-
 hydroxylamine
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.1.10 membrane firmly attached to mitochondrial membrane Rattus norvegicus 16020
-
1.7.1.10 mitochondrion firmly attached to membrane Rattus norvegicus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.10 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.1.10 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.10 hydroxylamine + NADH possible identity of hydroxylamine reductase and the enzyme which reduces anthranilic hydroxamic acid to the amide Rattus norvegicus NH3 + NAD+ + H2O
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.1.10 additional information
-
 NADH prevents heat damage Rattus norvegicus
1.7.1.10 40
-
 membrane bound enzyme, 10 min, pH 7.4, dithiothreitol, no loss of activity Rattus norvegicus
1.7.1.10 50
-
 membrane bound enzyme, 10 min, pH 7.4, dithiothreitol, 50% loss of activity Rattus norvegicus
1.7.1.10 60
-
 membrane bound enzyme, pH 7.4, dithiothreitol, complete loss of activity Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.7.1.10 5.4
-
 complete loss of activity after brief exposure Rattus norvegicus
1.7.1.10 8.4
-
 no loss of activity after brief exposure Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.10 NADH
-
 Rattus norvegicus