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Literature summary extracted from

  • Becana, M.; Klucas, R.V.
    Enzymatic and nonenzymatic mechanisms for ferric leghemoglobin reduction in legume root nodules (1990), Proc. Natl. Acad. Sci. USA, 87, 7295-7299.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.6 Amobarbital 1 mM, 35% after 0.5-1 h, 80% after 4 h Glycine max
1.6.2.6 catalase possibly H2O2 as reaction intermediate Glycine max
1.6.2.6 iodoacetamide 1 mM, 95-100% inhibition Glycine max
1.6.2.6 nicotinate ferrileghemoglobin-nitrite complex Glycine max
1.6.2.6 p-hydroxymercuribenzoate 0.5 mM, slight inhibition Glycine max
1.6.2.6 Quinacrine 0.5 mM, 30-40% inhibition Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.6 0.0133
-
ferrileghemoglobin
-
Phaseolus vulgaris
1.6.2.6 0.0133
-
ferrileghemoglobin
-
Vigna unguiculata
1.6.2.6 0.088
-
ferrileghemoglobin
-
Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.6 55000
-
2 * 55000, SDS-PAGE Glycine max
1.6.2.6 55000
-
2 * 55000, SDS-PAGE Vigna unguiculata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.2.6 NAD(P)H + ferrileghemoglobin Glycine max
-
NAD(P)+ + ferroleghemoglobin
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.6 Glycine max
-
soybean
-
1.6.2.6 Phaseolus vulgaris
-
bean
-
1.6.2.6 Vigna unguiculata
-
cowpea
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.6 root nodule
-
Phaseolus vulgaris
-
1.6.2.6 root nodule
-
Glycine max
-
1.6.2.6 root nodule
-
Vigna unguiculata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.6 0.215
-
reduction of ferrileghemoglobin Glycine max
1.6.2.6 2
-
reduction of 2,6-dichlorophenolindophenol Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.6 NAD(P)H + ferrileghemoglobin
-
Glycine max NAD(P)+ + ferroleghemoglobin
-
?
1.6.2.6 NAD(P)H + ferrileghemoglobin
-
Vigna unguiculata NAD(P)+ + ferroleghemoglobin
-
?
1.6.2.6 NADH + H+ + ferrileghemoglobin
-
Phaseolus vulgaris NAD+ + ferroleghemoglobin
-
r

Subunits

EC Number Subunits Comment Organism
1.6.2.6 dimer 2 * 55000, SDS-PAGE Glycine max
1.6.2.6 dimer 2 * 55000, SDS-PAGE Vigna unguiculata

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.6 NADH
-
Phaseolus vulgaris
1.6.2.6 NADH
-
Glycine max
1.6.2.6 NADH
-
Vigna unguiculata
1.6.2.6 NADPH 54-80% of NADH activity Phaseolus vulgaris
1.6.2.6 NADPH 54-80% of NADH activity Glycine max
1.6.2.6 NADPH 54-80% of NADH activity Vigna unguiculata