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Literature summary extracted from

  • Yanagawa, H.; Egami, F.
    Asparagusate dehydrogenases and lipoyl dehydrogenase from Asparagus mitochondria. Physical, chemical, and enzymatic properties (1976), J. Biol. Chem., 251, 3637-3644.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.1.11 lecithin drastic activation of lipoyl dehydrogenase activity, no activation of asparagusate dehydrogenase activity, enzyme II Asparagus officinalis
1.8.1.11 NAD+ reduction of lipoic acid is activated Asparagus officinalis
1.8.1.11 Tween 80 drastic activation of lipoyl dehydrogenase activity, no activation of asparagusate dehydrogenase activity, enzyme II Asparagus officinalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.11 arsenite low inhibition Asparagus officinalis
1.8.1.11 asparagusic acid inhibits lipoyl dehydrogenase activity Asparagus officinalis
1.8.1.11 cetyltrimethyl ammonium bromide affects lipoyl dehydrogenase activity Asparagus officinalis
1.8.1.11 FAD strongly inhibits the two reductase activities Asparagus officinalis
1.8.1.11 Hg2+ inhibitory Asparagus officinalis
1.8.1.11 iodoacetamide low inhibition Asparagus officinalis
1.8.1.11 additional information inhibitors indicate an involvement of protein disulfide linkage or thiol group in the catalytic site Asparagus officinalis
1.8.1.11 N-ethylmaleimide
-
 Asparagus officinalis
1.8.1.11 NADH high concentrations, increase of inhibitory effect by addition of arsenite Asparagus officinalis
1.8.1.11 p-chloromercuribenzoic acid
-
 Asparagus officinalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.11 0.8
-
 potassium ferricyanide enzyme II Asparagus officinalis
1.8.1.11 0.9
-
 potassium ferricyanide enzyme I Asparagus officinalis
1.8.1.11 3
-
 lipoic acid
-
 Asparagus officinalis
1.8.1.11 3 3.3 lipoate enzyme I and II Asparagus officinalis
1.8.1.11 20
-
 asparagusic acid
-
 Asparagus officinalis
1.8.1.11 20 21.5 Asparagusate enzyme I and II Asparagus officinalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.11 mitochondrion
-
 Asparagus officinalis 5739
-
1.8.1.11 soluble
-
 Asparagus officinalis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.11 110000 111000 ultracentrifugation Asparagus officinalis
1.8.1.11 112000
-
 calculation of amino acid content Asparagus officinalis
1.8.1.11 112000
-
 enzyme I and II, gel filtration Asparagus officinalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.11 asparagusate + NADH Asparagus officinalis
-
 dihydroasparagusate + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.11 Asparagus officinalis
-
 2 asparagusate dehydrogenases with lipoyl dehydrogenase activity
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.11 gel filtration, ion-exchange, ultracentrifugation, copurification of type I and II Asparagus officinalis

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.1.11 3-mercapto-2-mercaptomethylpropanoate + NAD+ = asparagusate + NADH + H+ exhibits lipoyl dehydrogenase activity Asparagus officinalis
a
1.8.1.11 3-mercapto-2-mercaptomethylpropanoate + NAD+ = asparagusate + NADH + H+ exhibits diaphorase activity Asparagus officinalis
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.11 shoot
-
 Asparagus officinalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.11 0.215
-
 enzyme/fraction II towards asparagusic acid Asparagus officinalis
1.8.1.11 0.425
-
 enzyme/fraction I towards asparagusic acid Asparagus officinalis
1.8.1.11 0.5464
-
 enzyme/fraction II towards lipoic acid Asparagus officinalis
1.8.1.11 1.082
-
 enzyme/fraction I towards lipoic acid Asparagus officinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.11 2 ferricyanide + NADH
-
 Asparagus officinalis 2 ferrocyanide + NAD+ + H+
-
 ?
1.8.1.11 asparagusate + NADH
-
 Asparagus officinalis dihydroasparagusate + NAD+
-
 r
1.8.1.11 asparagusic acid + NADH reverse reaction very slow Asparagus officinalis dihydroasparagusate + NAD+
-
 r
1.8.1.11 lipoic acid + NADH reverse reaction very slow Asparagus officinalis dihydrolipoic acid + NAD+
-
 r
1.8.1.11 NADH + H+ + potassium ferricyanide
-
 Asparagus officinalis NAD+ + potassium ferrocyanide
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.11 25
-
 assay at Asparagus officinalis
1.8.1.11 40 45
-
 Asparagus officinalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.1.11 50
-
 5 min, activity completely retained Asparagus officinalis
1.8.1.11 50
-
 above, gradual loss of lipoyl dehydrogenase activity, complete loss at 90°C Asparagus officinalis
1.8.1.11 60 70 5 min, 1.4-fold activation of asparagusate dehydrogenase activity Asparagus officinalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.11 5.25
-
 for K3Fe(CN)6 reduction Asparagus officinalis
1.8.1.11 5.9
-
 asparagusic and lipoic acid reduction Asparagus officinalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.11 FAD flavoprotein, approximately 1 mol of FAD per mol of protein Asparagus officinalis
1.8.1.11 NAD+ up to 0.2 mM, stimulates the reduction activity if NADH concentration is 0.2 mM Asparagus officinalis
1.8.1.11 NADH no replacement of NADH by NADPH Asparagus officinalis
1.8.1.11 NADH 0.1 mM optimal concentration for maximal activity Asparagus officinalis