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Literature summary extracted from

  • Missirlis, F.; Ulschmid, J.K.; Hirosawa-Takamori, M.; Gronke, S.; Schafer, U.; Becker, K.; Phillips, J.P.; Jackle, H.
    Mitochondrial and cytoplasmic thioredoxin reductase variants encoded by a single Drosophila gene are both essential for viability (2002), J. Biol. Chem., 277, 11521-11526.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.1.9 cysteine C-terminal redox active disulfide Drosophila melanogaster

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.9 expression of cytosolic and mitochondrial isoform as His-tagged enzymes in Escherichia coli Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.9 additional information His-tagged transcript specific mutants, varied N-terminus, 1 null-mutant Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.9 0.001
-
NADPH cytosolic and mitochondrial TrxR1 isoform Drosophila melanogaster
1.8.1.9 0.007
-
thioredoxin cytosolic TrxR1 isoform Drosophila melanogaster
1.8.1.9 0.007
-
thioredoxin-1 disulfide TrxR(cyto) Drosophila melanogaster
1.8.1.9 0.019
-
thioredoxin mitochondrial TrxR1 isoform Drosophila melanogaster
1.8.1.9 0.019
-
thioredoxin-1 disulfide TRxR(mito) Drosophila melanogaster
1.8.1.9 0.38
-
DTNB cytosolic TrxR1 isoform Drosophila melanogaster
1.8.1.9 0.41
-
DTNB mitochondrial TrxR1 isoform Drosophila melanogaster
1.8.1.9 1
-
NADPH TrxR-1(cyto) Drosophila melanogaster
1.8.1.9 1
-
NADPH TrxR-1(mito) Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.9 cytoplasm TrxR-1(cyto) Drosophila melanogaster 5737
-
1.8.1.9 cytosol isoform TrxR1 Drosophila melanogaster 5829
-
1.8.1.9 mitochondrion isoform TrxR3 and TrxR1 Drosophila melanogaster 5739
-
1.8.1.9 mitochondrion TrxR-1(mito) Drosophila melanogaster 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.1.9 additional information TrxR-1(cyto) is not a selenoprotein Drosophila melanogaster
1.8.1.9 additional information TrxR-1(mito) is not a selenoprotein Drosophila melanogaster

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.9 53200
-
cytosolic TrxR1 isoform, deduced from amino acid sequence Drosophila melanogaster
1.8.1.9 63700
-
mitochondrial TrxR1 isoform, deduced from amino acid sequence Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.9 thioredoxin + NADP+ Drosophila melanogaster defense against oxidative stress thioredoxin disulfide + NADPH
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.9 Drosophila melanogaster
-
-
-
1.8.1.9 Drosophila melanogaster
-
3 isoforms TrxR1, TrxR2, TrxR3, encoded by 3 genes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.9 recombinant wild-type cytosolic and mitochondrial isoforms and mutants from Escherichia coli Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
-
Drosophila melanogaster 2-nitro-5-thiobenzoate + NADP+
-
?
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH i.e. DTNB Drosophila melanogaster 2-nitro-5-thiobenzoate + NADP+
-
?
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH coupled assay Drosophila melanogaster 2-nitro-5-thiobenzoate + NADP+
-
?
1.8.1.9 thioredoxin + NADP+ coupled assay with DTNB Drosophila melanogaster thioredoxin disulfide + NADPH
-
?
1.8.1.9 thioredoxin + NADP+ defense against oxidative stress Drosophila melanogaster thioredoxin disulfide + NADPH
-
?
1.8.1.9 thioredoxin-1 disulfide + NADPH
-
Drosophila melanogaster NADP+ + thioredoxin-1
-
?
1.8.1.9 thioredoxin-1 disulfide + NADPH + H+
-
Drosophila melanogaster thioredoxin-1 + NADP+
-
r

Subunits

EC Number Subunits Comment Organism
1.8.1.9 ? x * 53200, TrxR-1(cyto), calculation from nucleotide sequence Drosophila melanogaster
1.8.1.9 ? x * 63700, TrxR-1(mito), calculation from nucleotide sequence Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
1.8.1.9 TrxR
-
Drosophila melanogaster
1.8.1.9 TrxR-1(cyto)
-
Drosophila melanogaster
1.8.1.9 TrxR-1(mito)
-
Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.9 25
-
assay at Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.9 7.1
-
cytosolic and mitochondrial TrxR1 isoform Drosophila melanogaster
1.8.1.9 7.1
-
TrxR-1(cyto) Drosophila melanogaster
1.8.1.9 7.1
-
TrxR-1(mito) Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.9 FAD
-
Drosophila melanogaster
1.8.1.9 NADPH
-
Drosophila melanogaster

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.8.1.9 Drosophila melanogaster TrxR-1(cyto)
-
5.93
1.8.1.9 Drosophila melanogaster TrxR-1(mito)
-
8.15