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Literature summary extracted from

  • Krug, M.; Straube, G.
    Degradation of phenolic compounds by the yeast Candida tropicalis HP 15. II. Some properties of the first two enzymes of the degradation pathway (1986), J. Basic Microbiol., 26, 271-281.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.14.13.7 longer exposure to ultrasound drastically reduces enzyme activity Candida tropicalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.1 dichlorocatechol
-
Candida tropicalis
1.13.11.1 gallic acid
-
Candida tropicalis
1.13.11.1 Vanillin
-
Candida tropicalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.7 0.0015
-
resorcinol crude extract Candida tropicalis
1.14.13.7 0.005
-
phenol crude extract Candida tropicalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.7 phenol + NADPH + O2 Candida tropicalis enzyme of phenol degradation pathway ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Candida tropicalis
-
HP 15
-
1.13.11.1 Candida tropicalis HP 15
-
HP 15
-
1.14.13.7 Candida tropicalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1 partially, two active fractions Candida tropicalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 4-chlorocatechol + O2
-
Candida tropicalis (2E,4Z)-3-chlorohexa-2,4-dienedioate
-
?
1.13.11.1 4-chlorocatechol + O2
-
Candida tropicalis HP 15 (2E,4Z)-3-chlorohexa-2,4-dienedioate
-
?
1.13.11.1 4-methylcatechol + O2
-
Candida tropicalis (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
?
1.13.11.1 4-methylcatechol + O2
-
Candida tropicalis HP 15 (2Z,4Z)-3-methylhexa-2,4-dienedioate
-
?
1.13.11.1 catechol + O2
-
Candida tropicalis cis,cis-muconate
-
?
1.13.11.1 catechol + O2
-
Candida tropicalis HP 15 cis,cis-muconate
-
?
1.13.11.1 hydroxyquinol + O2
-
Candida tropicalis maleylacetic acid
-
?
1.13.11.1 hydroxyquinol + O2
-
Candida tropicalis HP 15 maleylacetic acid
-
?
1.14.13.7 2-aminophenol + O2 + NADPH
-
Candida tropicalis ?
-
?
1.14.13.7 2-chlorophenol + O2 + NADPH
-
Candida tropicalis ?
-
?
1.14.13.7 2-methyl-phenol + O2 + NADPH i.e. o-cresol Candida tropicalis ?
-
?
1.14.13.7 3-aminophenol + O2 + NADPH
-
Candida tropicalis ?
-
?
1.14.13.7 3-chlorophenol + O2 + NADPH
-
Candida tropicalis ?
-
?
1.14.13.7 3-methylphenol + O2 + NADPH i.e. m-cresol Candida tropicalis ?
-
?
1.14.13.7 4-aminophenol + O2 + NADPH
-
Candida tropicalis ?
-
?
1.14.13.7 4-chlorocatechol + O2 + NADPH
-
Candida tropicalis ?
-
?
1.14.13.7 4-chlorophenol + O2 + NADPH
-
Candida tropicalis ?
-
?
1.14.13.7 4-methyl-phenol + O2 + NADPH i.e. p-cresol Candida tropicalis ?
-
?
1.14.13.7 catechol + O2 + NADPH
-
Candida tropicalis ?
-
?
1.14.13.7 metol + O2 + NADPH
-
Candida tropicalis ?
-
?
1.14.13.7 additional information broad specificity Candida tropicalis ?
-
?
1.14.13.7 additional information not: salicylic acid Candida tropicalis ?
-
?
1.14.13.7 orcinol + O2 + NADPH weak Candida tropicalis ?
-
?
1.14.13.7 phenol + NADPH + O2
-
Candida tropicalis catechol + NADP+ + H2O
-
?
1.14.13.7 phenol + NADPH + O2 enzyme of phenol degradation pathway Candida tropicalis ?
-
?
1.14.13.7 phloroglucinol + O2 + NADPH
-
Candida tropicalis ?
-
?
1.14.13.7 quinol + O2 + NADPH
-
Candida tropicalis 1,2,4-trihydroxybenzene + NADP+ + H2O
-
?
1.14.13.7 resorcinol + NADPH + O2
-
Candida tropicalis ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.7 30
-
enzyme from resorcinol grown cells Candida tropicalis
1.14.13.7 40
-
enzyme from phenol-induced cells Candida tropicalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.7 4
-
stable for some hours Candida tropicalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 7.5 9.6
-
Candida tropicalis
1.14.13.7 7.6 8
-
Candida tropicalis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.7 7 8.7 about 50% of activity maximum at pH 7.0 and 8.7 Candida tropicalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.7 FAD activates Candida tropicalis
1.14.13.7 NADPH
-
Candida tropicalis