EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.12.98.1 | FAD | reactivation without added FAD restores only methyl viologen-dependent activity, no activity with F420 | Methanobacterium formicicum | |
1.12.98.1 | KCl | 0.8 M activate F420-linked activity more than 10fold, slight inhibition of methyl viologen linked activity | Methanobacterium formicicum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.12.98.1 | 0.037 | - |
coenzyme F420 | - |
Methanobacterium formicicum | |
1.12.98.1 | 0.08 | - |
coenzyme F0 | - |
Methanobacterium formicicum | |
1.12.98.1 | 0.087 | - |
FAD | - |
Methanobacterium formicicum | |
1.12.98.1 | 1.56 | - |
methyl viologen | - |
Methanobacterium formicicum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.12.98.1 | Iron | - |
Methanobacterium formicicum | |
1.12.98.1 | Nickel | - |
Methanobacterium formicicum | |
1.12.98.1 | selenium | no selenium detected | Methanobacterium formicicum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.12.98.1 | 102000 | - |
smallest active species, gel filtration | Methanobacterium formicicum |
1.12.98.1 | 109000 | - |
gel filtration | Methanobacterium formicicum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.98.1 | H2 + coenzyme F420 | Methanobacterium formicicum | enzyme of methanogenesis pathway | reduced coenzyme F420 | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.12.98.1 | Methanobacterium formicicum | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.12.98.1 | purified enzyme requires reductive reactivation before assay, reactivation possible with H2 or glucose and glucose oxidase | Methanobacterium formicicum |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.12.98.1 | - |
Methanobacterium formicicum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.12.98.1 | 97.6 | - |
purified enzyme with F420 | Methanobacterium formicicum |
1.12.98.1 | 98.6 | - |
purified enzyme with F0 | Methanobacterium formicicum |
1.12.98.1 | 172 | - |
purified enzyme with methyl viologen | Methanobacterium formicicum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.98.1 | H2 + coenzyme F0 | - |
Methanobacterium formicicum | reduced coenzyme F0 | - |
? | |
1.12.98.1 | H2 + coenzyme F420 | - |
Methanobacterium formicicum | reduced coenzyme F420 | - |
r | |
1.12.98.1 | H2 + coenzyme F420 | enzyme of methanogenesis pathway | Methanobacterium formicicum | reduced coenzyme F420 | - |
r | |
1.12.98.1 | H2 + FAD | - |
Methanobacterium formicicum | H+ + FADH2 | - |
r | |
1.12.98.1 | H2 + methyl viologen | - |
Methanobacterium formicicum | ? | - |
? | |
1.12.98.1 | additional information | reduces different flavins | Methanobacterium formicicum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.12.98.1 | multimer | alpha,beta,gamma 43600 + 36700 + 28000, SDS-PAGE, exact subunit composition not known, ratio 1:1:1 postulated | Methanobacterium formicicum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.12.98.1 | 55 | - |
- |
Methanobacterium formicicum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.12.98.1 | 7 | 7.5 | - |
Methanobacterium formicicum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.12.98.1 | 5.5 | 9.5 | - |
Methanobacterium formicicum |
1.12.98.1 | 5.5 | 8 | reverse reaction | Methanobacterium formicicum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.12.98.1 | FAD | 1 mol per mol 109000 Da species | Methanobacterium formicicum | |
1.12.98.1 | FAD | FAD depleted enzyme reduces methyl viologen but not F420 | Methanobacterium formicicum | |
1.12.98.1 | additional information | no activity with FMN after FAD depletion | Methanobacterium formicicum |