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Literature summary extracted from

  • Michel, R.; Massanz, C.; Kostka, S.; Richter, M.; Fiebig, K.
    Biochemical characterization of the 8-hydroxy-5-deazaflavin-reactive hydrogenase from Methanosarcina barkeri Fusaro (1995), Eur. J. Biochem., 233, 727-735.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.98.1 0.003
-
H2 37°C, pH 7.2, cosubstrate: oxidized coenzyme F420 Methanosarcina barkeri
1.12.98.1 0.004
-
H2 37°C, pH 7.2, cosubstrate: oxidized methyl viologen Methanosarcina barkeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.98.1 Iron 1 mol of the 198000 Da enzyme form contains 28-32 mol of non-heme iron Methanosarcina barkeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.98.1 30000
-
2 * 48000 (alpha) + 2 * 33000 (beta) + 2 * 30000 (gamma) Methanosarcina barkeri
1.12.98.1 33000
-
2 * 48000 (alpha) + 2 * 33000 (beta) + 2 * 30000 (gamma) Methanosarcina barkeri
1.12.98.1 48000
-
2 * 48000 (alpha) + 2 * 33000 (beta) + 2 * 30000 (gamma) Methanosarcina barkeri
1.12.98.1 198000
-
-
Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.98.1 H2 + oxidized coenzyme F420 Methanosarcina barkeri
-
reduced coenzyme F420
-
?
1.12.98.1 H2 + oxidized coenzyme F420 Methanosarcina barkeri DSM 804
-
reduced coenzyme F420
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.1 Methanosarcina barkeri P80490 and P80489 and P80491 P80490: subunit beta, P80489: subunit alpha, P80491: subunit gamma
-
1.12.98.1 Methanosarcina barkeri DSM 804 P80490 and P80489 and P80491 P80490: subunit beta, P80489: subunit alpha, P80491: subunit gamma
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.98.1
-
Methanosarcina barkeri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.98.1 82.8
-
37°C, pH 7.2, cosubstrate: oxidized coenzyme F420 Methanosarcina barkeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.1 H2 + oxidized benzyl viologen
-
Methanosarcina barkeri reduced benzyl viologen
-
?
1.12.98.1 H2 + oxidized benzyl viologen
-
Methanosarcina barkeri DSM 804 reduced benzyl viologen
-
?
1.12.98.1 H2 + oxidized coenzyme F0 cofactor F0 i.e. 7,8-didemethy1-8-hydroxy-S-deazaflavin Methanosarcina barkeri reduced coenzyme F20
-
?
1.12.98.1 H2 + oxidized coenzyme F0 cofactor F0 i.e. 7,8-didemethy1-8-hydroxy-S-deazaflavin Methanosarcina barkeri DSM 804 reduced coenzyme F20
-
?
1.12.98.1 H2 + oxidized coenzyme F420
-
Methanosarcina barkeri reduced coenzyme F420
-
?
1.12.98.1 H2 + oxidized coenzyme F420
-
Methanosarcina barkeri DSM 804 reduced coenzyme F420
-
?

Subunits

EC Number Subunits Comment Organism
1.12.98.1 hexamer 2 * 48000 (alpha) + 2 * 33000 (beta) + 2 * 30000 (gamma) Methanosarcina barkeri

Synonyms

EC Number Synonyms Comment Organism
1.12.98.1 8-hydroxy-5-deazaflavin-reactive hydrogenase
-
Methanosarcina barkeri
1.12.98.1 F420-reducing hydrogenase
-
Methanosarcina barkeri
1.12.98.1 Mbar_A0449 subunit beta Methanosarcina barkeri
1.12.98.1 Mbar_A0450 subunit gamma Methanosarcina barkeri
1.12.98.1 Mbar_A0452 subunit alpha Methanosarcina barkeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.12.98.1 55
-
-
Methanosarcina barkeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.98.1 6.5 7.25 deazaflavin reducing activity Methanosarcina barkeri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.12.98.1 5.5 10 the methylviologen-reducing activity increased with pH from pH 5.5-10 Methanosarcina barkeri

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.98.1 FAD 1 mol of the 198000 Da enzyme form contains 2 mol of FAD Methanosarcina barkeri

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.12.98.1 Methanosarcina barkeri isoelectric focusing
-
5.3

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.12.98.1 25000
-
H2 37°C, pH 7.2, cosubstrate: oxidized coenzyme F420 Methanosarcina barkeri
1.12.98.1 69000
-
H2 37°C, pH 7.2, cosubstrate: oxidized methyl viologen Methanosarcina barkeri