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Literature summary extracted from

  • Lindblad, B.; Lindstedt, G.; Lindstedt, S.; Rundgren, M.
    Purification and some properties of human 4-hydroxyphenylpyruvate dioxygenase (I) (1977), J. Biol. Chem., 252, 5073-5084.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.27 ascorbate reducing cofactor required, either ascorbate, or a combination of 2,6-dichlorophenol indophenol and glutathione in presence of catalase Homo sapiens
1.13.11.27 glutathione reducing cofactor required, either ascorbate, or a combination of 2,6-dichlorophenol indophenol and glutathione in presence of catalase Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.27 1,10-phenanthroline 0.01 mM, 50% inhibition Homo sapiens
1.13.11.27 1-(2'-thenonyl)-3,3,3-trifluoroacetone 0.0049 mM, 50% inhibition Homo sapiens
1.13.11.27 2,2'-bipyridyl 1.8 mM, 50% inhibition Homo sapiens
1.13.11.27 4-aminophenylpyruvate competitive Homo sapiens
1.13.11.27 bathophenanthroline 0.018 mM, 50% inhibition; reactivation to 30% by dialysis and to 60% by addition to Fe2+ Homo sapiens
1.13.11.27 Cupferron 0.0011 mM, 50% inhibition Homo sapiens
1.13.11.27 diethyldithiocarbamate 0.0049 mM, 50% inhibition; restored by dialysis Homo sapiens
1.13.11.27 Dithizone 0.14 mM, 50% inhibition Homo sapiens
1.13.11.27 Fe2+ 1 mM, 30% inhibition Homo sapiens
1.13.11.27 H2O2 10 nM, 60% inhibition. 0.001 mM, complete inhibition Homo sapiens
1.13.11.27 phenylpyruvate competitive Homo sapiens
1.13.11.27 YCl3 1 mM, 50% inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.27 0.03
-
 4-hydroxyphenylpyruvate
-
 Homo sapiens
1.13.11.27 0.05
-
 O2
-
 Homo sapiens
1.13.11.27 0.5
-
 phenylpyruvate
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.27 43000
-
 2 * 43000, SDS-PAGE Homo sapiens
1.13.11.27 66000
-
 gel filtration Homo sapiens
1.13.11.27 87000
-
 equilibrium sedimentation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.27 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.27
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.27 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.27 0.53
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.13.11.27 -60°C, pH 7.5, 3 months, 20% loss of activity when enzyme concentration is 0.5 g/L in Tris/HCl buffer, no loss of activity after 4 years when enzyme concentration is 5 g/l Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.27 4-hydroxyphenylpyruvate + O2 activity with the keto-form of 4-hydroxyphenylpyruvate is 40times higher than with the enol tautomer Homo sapiens homogentisate + CO2
-
 ?
1.13.11.27 phenylpyruvate + O2
-
 Homo sapiens 2-hydroxyphenylacetate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.27 dimer 2 * 43000, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.27 4.5
-
 and a second optimum at pH 7.8 Homo sapiens
1.13.11.27 7.8
-
 and a second optimum at pH 4.5 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.27 2.5 10 37°C, 30 min, in presence of Fe2+, stable Homo sapiens