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Literature summary extracted from

  • Garcia, I.; Rodgers, M.; Pepin, R.; Hsieh, T.F.; Matringe, M.
    Characterization and subcellular compartmentation of recombinant 4-hydroxyphenylpyruvate dioxygenase from Arabidopsis in transgenic tobacco (1999), Plant Physiol., 119, 1507-1516.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.27 overproduction of the recombinant enzyme in Escherichia coli JM105, overexpression of the complete coding sequence in transgenic Nicotiana tabacum using Agrobacterium tumefaciens transformation Arabidopsis sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.27 cytosol
-
 Arabidopsis sp. 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.27 100000
-
 non-denaturing PAGE Arabidopsis sp.

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.27 Arabidopsis sp.
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.13.11.27 4-hydroxyphenylpyruvate + O2 = homogentisate + CO2 in plants this enzyme activity is involved in two distinct metabolic processes, the biosynthesis of prenylquinones and the catabolism of tyrosine Arabidopsis sp.
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.27 leaf photosynthetically active tissue Arabidopsis sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.27 4-hydroxyphenylpyruvate + O2
-
 Arabidopsis sp. homogentisate + CO2
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.27 6.5
-
-
 Arabidopsis sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.27 5.5 7.7 pH 5.5: about 50% of maximal activity, pH 7.7: about 55% of maximal activity Arabidopsis sp.