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Literature summary extracted from

  • Flatman, S.; Hurst, J.S.; McDonald-Gibson, R.G.; Jonas, G.E.G.; Slater, T.F.
    Biochemical studies on a 12-lipoxygenase in human uterine cervix (1986), Biochim. Biophys. Acta, 883, 7-14.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.31 ATP 2 mM ATP + 1 mM CaCl2 stimulate approximately 2fold Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.31 caffeic acid 0.01 mM, 20-30% inhibition Homo sapiens
1.13.11.31 eicosatetraynoic acid 0.01 mM, 90-95% inhibition Homo sapiens
1.13.11.31 esculetin 0.01 mM, 90-95% inhibition Homo sapiens
1.13.11.31 iodoacetate 1 mM, 5% inhibition Homo sapiens
1.13.11.31 Lubrol 0.2%, 60% inhibition Homo sapiens
1.13.11.31 NEM 1 mM, 15% inhibition Homo sapiens
1.13.11.31 nordihydroguaiaretic acid 0.01 mM, 90-95% inhibition Homo sapiens
1.13.11.31 p-hydroxymercuribenzoate 1 mM, 75% inhibition Homo sapiens
1.13.11.31 Renex
-
 Homo sapiens
1.13.11.31 Triton X-100
-
 Homo sapiens
1.13.11.31 Tween 20
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.31 0.015
-
 arachidonic acid
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.31 Ca2+ 2 mM ATP + 1 mM CaCl2 stimulate approximately 2fold Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.31 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.31
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.31 uterine cervix
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.31 additional information
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
1.13.11.31 -20°C, remains stable for 6 weeks Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.31 arachidonate + O2 i.e. 5Z,8Z,11Z,14Z-eicosatetraenoic acid Homo sapiens (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.31 4
-
 90% loss of activity after 4 h Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.31 6.5
-
-
 Homo sapiens