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Literature summary extracted from

  • Lalla-Maharajh, W.V.; Hall, D.O.; Cammack, R.; Rao, K.K.
    Purification and properties of the membrane-bound by hydrogenase from Desulfovibrio desulfuricans (1983), Biochem. J., 209, 445-454.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.2.1 CO
-
 Desulfovibrio desulfuricans
1.12.2.1 KI above 0.5 M, considerable inhibition of H2 evolution Desulfovibrio desulfuricans
1.12.2.1 N-bromosuccinimide 0.07 mM, complete loss of activity Desulfovibrio desulfuricans
1.12.2.1 NaCl above 0.5 M, considerable inhibition of H2 evolution Desulfovibrio desulfuricans
1.12.2.1 NaF above 0.5 M, considerable inhibition of H2 evolution Desulfovibrio desulfuricans
1.12.2.1 O2 strong inhibition of H2 evolution from reduced methyl viologen Desulfovibrio desulfuricans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.12.2.1 0.002
-
 ferrocytochrome c3 H2-evolution assay Desulfovibrio desulfuricans
1.12.2.1 0.003
-
 reduced methyl viologen H2-evolution assay Desulfovibrio desulfuricans
1.12.2.1 0.08
-
 reduced benzyl viologen H2-evolution assay Desulfovibrio desulfuricans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.2.1 membrane bound to Desulfovibrio desulfuricans 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.2.1 Iron contains 6 iron atoms and six acid-labile sulfur groups per molecule, presence of [4Fe-4S]3+ or [3Fe-3S]3+ Desulfovibrio desulfuricans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.2.1 58000
-
 gel filtration Desulfovibrio desulfuricans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.2.1 additional information Desulfovibrio desulfuricans cytochrome c3 is the natural electron acceptor ?
-
 ?
1.12.2.1 additional information Desulfovibrio desulfuricans Norway cytochrome c3 is the natural electron acceptor ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.12.2.1 Desulfovibrio desulfuricans
-
 Norway
-
1.12.2.1 Desulfovibrio desulfuricans Norway
-
 Norway
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.2.1 membrane-bound enzyme Desulfovibrio desulfuricans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.2.1 70
-
-
 Desulfovibrio desulfuricans

Storage Stability

EC Number Storage Stability Organism
1.12.2.1 4°C, atmosphere of N2, air or H2, very little loss of activity after 4 weeks Desulfovibrio desulfuricans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.2.1 H+ + neutral red
-
 Desulfovibrio desulfuricans H2 + oxidized neutral red
-
 ?
1.12.2.1 H+ + neutral red
-
 Desulfovibrio desulfuricans Norway H2 + oxidized neutral red
-
 ?
1.12.2.1 H+ + phenosafranine
-
 Desulfovibrio desulfuricans H2 + oxidized phenosafranine
-
 ?
1.12.2.1 H+ + reduced methyl viologen weak activity in H2-uptake assay Desulfovibrio desulfuricans H2 + methyl viologen
-
 r
1.12.2.1 H2 + benzyl viologen
-
 Desulfovibrio desulfuricans H+ + reduced benzyl viologen
-
 r
1.12.2.1 H2 + benzyl viologen
-
 Desulfovibrio desulfuricans Norway H+ + reduced benzyl viologen
-
 r
1.12.2.1 H2 + ferricytochrome c3
-
 Desulfovibrio desulfuricans H+ + ferrocytochrome c3
-
 r
1.12.2.1 H2 + ferricytochrome c3
-
 Desulfovibrio desulfuricans Norway H+ + ferrocytochrome c3
-
 r
1.12.2.1 H2 + methylene blue no activity in H2-evolution assay Desulfovibrio desulfuricans H+ + reduced methylene blue
-
 ir
1.12.2.1 H2 + methylene blue no activity in H2-evolution assay Desulfovibrio desulfuricans Norway H+ + reduced methylene blue
-
 ir
1.12.2.1 additional information cytochrome c3 is the natural electron acceptor Desulfovibrio desulfuricans ?
-
 ?
1.12.2.1 additional information cytochrome c3 is the natural electron acceptor Desulfovibrio desulfuricans Norway ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.2.1 monomer 1 * 58000, SDS-PAGE Desulfovibrio desulfuricans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.12.2.1 4
-
 4 weeks, atmosphere of N2, air or H2, very little loss of activity Desulfovibrio desulfuricans
1.12.2.1 23
-
 atmosphere of N2, air or H2, 40% loss of activity after 2 weeks, complete loss of activity after 4 weeks Desulfovibrio desulfuricans
1.12.2.1 77
-
 stable below, 2 h Desulfovibrio desulfuricans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.2.1 6.5
-
 H2-evolution assay Desulfovibrio desulfuricans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.12.2.1 5 9 pH 5.0: about 30% of maximal activity, pH 9.0: about 35% of maximal activity Desulfovibrio desulfuricans

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.2.1 cytochrome c3
-
 Desulfovibrio desulfuricans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.12.2.1 additional information
-
 additional information Ki for CO is 1.64 kPa Desulfovibrio desulfuricans