Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pundir, C.S.; Garg, G.K.; Rathore, V.S.
    Purification and properties of indole 2,3-dioxygenase from maize leaves (1984), Phytochemistry, 23, 2423-2427.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.13.11.17 pH 7, 0.02 M sodium phosphate buffer, 0.1 mM EDTA, 6 h in the cold, slight loss of activity Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.17 2-mercaptoethanol 0.5 mM, 80% inhibition, Cu2+ protects Zea mays
1.13.11.17 Cys 0.5 mM, 78% inhibition, Cu2+ protects Zea mays
1.13.11.17 diethyldithiocarbamate reversed by Cu2+ Zea mays
1.13.11.17 Dithionite 1 mM, almost complete inhibition Zea mays
1.13.11.17 Fe2+ 0.5 mM, slight inhibition Zea mays
1.13.11.17 Fe3+ 0.5 mM, slight inhibition Zea mays
1.13.11.17 GSH 0.5 mM, 81% inhibition, Cu2+ protects Zea mays
1.13.11.17 Hg2+ 0.5 mM, slight inhibition Zea mays
1.13.11.17 NEM 0.5 mM, 34% inhibition Zea mays
1.13.11.17 Salicylaldoxime 0.5 mM, 34% inhibition Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.17 0.14
-
 indole
-
 Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.17 Cu2+ stimulation Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.17 98000
-
 gel filtration Zea mays

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.17 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.17
-
 Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.17 epicotyl
-
 Zea mays
-
1.13.11.17 leaf middle leaves Zea mays
-
1.13.11.17 shoot
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.17 0.0441
-
 disappearance of 1 nmol indole in 1 min Zea mays

Storage Stability

EC Number Storage Stability Organism
1.13.11.17 4°C, stable for 2 weeks Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.17 indole + O2 4 atoms of oxygen utilized in disappearance of 1 mol of indole, 2 atoms of oxygen required for initial cleavage of indole and one each for the oxidation of o-aminobenzaldehyde to anthranil and anthranilic acid, simultaneously Zea mays anthranilic acid
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.17 45
-
-
 Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.17 5
-
-
 Zea mays

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.17 3 6 stable Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.17 FAD slight stimulation Zea mays