EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.7.1.3 | o-phenanthroline | 40% increase of activity at 2.5 mM | Neurospora crassa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.7.1.3 | 8-hydroxyquinoline | 94% inhibition at 10 mM | Neurospora crassa | |
1.7.1.3 | azide | 96% inhibition at 5 mM | Neurospora crassa | |
1.7.1.3 | cyanide | - |
Neurospora crassa | |
1.7.1.3 | EDTA | - |
Neurospora crassa | |
1.7.1.3 | additional information | protection of inactivation by FAD and restorage by dithiothreitol | Neurospora crassa | |
1.7.1.3 | p-hydroxymercuribenzoate | reversed by sulfhydryl reagents | Neurospora crassa | |
1.7.1.3 | Phenylglyoxal | in 0.1 M phosphate, pH 7.3, 4 mM, inactivation after 15 min to 40% and to 20% after 60 min | Neurospora crassa |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.7.1.3 | Molybdenum | 1 mol per mol protein | Neurospora crassa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.7.1.3 | 115000 | - |
1 * 130000 + 1 * 115000, SDS-PAGE, homodimer of 2 * 150000 suggested | Neurospora crassa |
1.7.1.3 | 130000 | - |
1 * 130000 + 1 * 115000, SDS-PAGE, homodimer of 2 * 150000 suggested | Neurospora crassa |
1.7.1.3 | 150000 | - |
1 * 130000 + 1 * 115000, SDS-PAGE, homodimer of 2 * 150000 suggested | Neurospora crassa |
1.7.1.3 | 230000 | - |
sucrose density gradient, gel filtration | Neurospora crassa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.1.3 | nitrate + NADPH | Neurospora crassa | first step in the reduction of nitrate to ammonia, biosynthesis of amino acids and other nitrogen-containing cell constituents | nitrite + NADP+ + H2O | - |
? | |
1.7.1.3 | nitrate + NADPH | Neurospora crassa STA4 | first step in the reduction of nitrate to ammonia, biosynthesis of amino acids and other nitrogen-containing cell constituents | nitrite + NADP+ + H2O | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.7.1.3 | Ethanol | mild denaturation | Neurospora crassa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.7.1.3 | Neurospora crassa | - |
STA4 | - |
1.7.1.3 | Neurospora crassa STA4 | - |
STA4 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.7.1.3 | streptomycin sulfate and ammonium sulfate precipitation, ion-exchange, gel filtration, isoelectric focusing, FAD-affinity | Neurospora crassa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.7.1.3 | mycelium | - |
Neurospora crassa | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.7.1.3 | additional information | - |
- |
Neurospora crassa |
EC Number | Storage Stability | Organism |
---|---|---|
1.7.1.3 | -10°C, 50 mM sodium phosphate, pH 6.9, 30% glycerol, 0.5 mM EDTA, 0.5 mM dithiothreitol, 0.5 mM phenylmethylsulfonylfluoride, 0.1 mM FAD, stable for at least 6 months | Neurospora crassa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.1.3 | nitrate + NADPH | 4 activities: NADPH-nitrate reductase, FADH-nitrate reductase, reduced methyl viologen-nitrate reductase and NADPH-cytochrome c reductase | Neurospora crassa | nitrite + NADP+ + H2O | - |
? | |
1.7.1.3 | nitrate + NADPH | first step in the reduction of nitrate to ammonia, biosynthesis of amino acids and other nitrogen-containing cell constituents | Neurospora crassa | nitrite + NADP+ + H2O | - |
? | |
1.7.1.3 | nitrate + NADPH | 4 activities: NADPH-nitrate reductase, FADH-nitrate reductase, reduced methyl viologen-nitrate reductase and NADPH-cytochrome c reductase | Neurospora crassa STA4 | nitrite + NADP+ + H2O | - |
? | |
1.7.1.3 | nitrate + NADPH | first step in the reduction of nitrate to ammonia, biosynthesis of amino acids and other nitrogen-containing cell constituents | Neurospora crassa STA4 | nitrite + NADP+ + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.7.1.3 | dimer | 1 * 130000 + 1 * 115000, SDS-PAGE, homodimer of 2 * 150000 suggested | Neurospora crassa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.7.1.3 | cytochrome b557 | heme-iron, 2 mol per mol enzyme | Neurospora crassa | |
1.7.1.3 | FAD | 1 mol per subunit is assumed | Neurospora crassa |