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Literature summary extracted from
- Molecular and enzymatic properties of cytochrome aa3-type terminal oxidase derived from Nitrobacter agilis (1981), J. Biochem., 89, 265-273.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.9 | CN- | 0.001 mM, 50% inhibition | Nitrobacter winogradskyi |  |
| 7.1.1.9 | phosphate | - |
Nitrobacter winogradskyi | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.1.1.9 | 0.027 | - |
ferrocytochrome c | Candida krusei cytochrome c | Nitrobacter winogradskyi | |
| 7.1.1.9 | 0.029 | - |
ferrocytochrome c | Saccharomyces oviformis cytochrome c | Nitrobacter winogradskyi | |
| 7.1.1.9 | 0.053 | - |
ferrocytochrome c | tuna cytochrome c | Nitrobacter winogradskyi | |
| 7.1.1.9 | 0.11 | - |
ferrocytochrome c | horse cytochrome c | Nitrobacter winogradskyi | |
| 7.1.1.9 | 0.12 | - |
ferrocytochrome c | cow cytochrome c | Nitrobacter winogradskyi | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.9 | Cu | - |
Nitrobacter winogradskyi | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 7.1.1.9 | 31000 | - |
1 * 51000 + 1 * 31000, SDS-PAGE, Ferguson plot | Nitrobacter winogradskyi |
| 7.1.1.9 | 51000 | - |
1 * 51000 + 1 * 31000, SDS-PAGE, Ferguson plot | Nitrobacter winogradskyi |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.1.1.9 | Nitrobacter winogradskyi | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.1.1.9 | Triton X-100, DEAE-cellulose, Sephadex G-150 | Nitrobacter winogradskyi |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 7.1.1.9 | 114 | - |
oxidation of Candida krusei cytochrome c | Nitrobacter winogradskyi |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 7.1.1.9 | 0°C, up to 7 days | Nitrobacter winogradskyi |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.1.9 | ferrocytochrome c + O2 | Nitrobacter agilis ferrocytochrome c552 | Nitrobacter winogradskyi | ferricytochrome c + H2O | - |
? | |
| 7.1.1.9 | ferrocytochrome c + O2 | Saccharomyces oviformis cytochrome c | Nitrobacter winogradskyi | ferricytochrome c + H2O | - |
? | |
| 7.1.1.9 | ferrocytochrome c + O2 | horse ferrocytochrome c | Nitrobacter winogradskyi | ferricytochrome c + H2O | - |
? | |
| 7.1.1.9 | ferrocytochrome c + O2 | cow cytochrome c | Nitrobacter winogradskyi | ferricytochrome c + H2O | - |
? | |
| 7.1.1.9 | ferrocytochrome c + O2 | tuna cytochrome c | Nitrobacter winogradskyi | ferricytochrome c + H2O | - |
? | |
| 7.1.1.9 | ferrocytochrome c + O2 | Candida krusei ferrocytochrome c | Nitrobacter winogradskyi | ferricytochrome c + H2O | - |
? | |
| 7.1.1.9 | ferrocytochrome c + O2 + H+ | - |
Nitrobacter winogradskyi | ferricytochrome c + H2O | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.1.1.9 | dimer | 1 * 51000 + 1 * 31000, SDS-PAGE, Ferguson plot | Nitrobacter winogradskyi |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.1.1.9 | 80 | - |
ferrocytochrome c | horse cytochrome c | Nitrobacter winogradskyi | |
| 7.1.1.9 | 90 | - |
ferrocytochrome c | cow cytochrome c | Nitrobacter winogradskyi | |
| 7.1.1.9 | 160 | - |
ferrocytochrome c | Saccharomyces oviformis cytochrome c | Nitrobacter winogradskyi | |
| 7.1.1.9 | 260 | - |
ferrocytochrome c | tuna cytochrome c | Nitrobacter winogradskyi | |
| 7.1.1.9 | 290 | - |
ferrocytochrome c | Candida krusei cytochrome c | Nitrobacter winogradskyi | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.1.1.9 | 5.6 | - |
oxidation of Candida krusei and Saccharomyces oviformis cytochrome c | Nitrobacter winogradskyi |
| 7.1.1.9 | 5.8 | - |
oxidation of tuna cytochrome c | Nitrobacter winogradskyi |
| 7.1.1.9 | 5.9 | - |
oxidation of horse and cow cytochrome c | Nitrobacter winogradskyi |
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Release 2023.1 (January 2023)
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