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Literature summary extracted from
- Isolation and partial characterization of the cytochrome oxidase from Rhodopseudomonas palustris (1976), Eur. J. Biochem., 68, 5-12.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 7.1.1.9 | glycerol: no stabilization during storage | Rhodopseudomonas palustris |
| 7.1.1.9 | inactivation by repeated freezing/thawing | Rhodopseudomonas palustris |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.1.9 | azide | 0.007 mM, 50% inhibition, 1 mM, complete inhibition | Rhodopseudomonas palustris |  |
| 7.1.1.9 | CN- | 0.0005 mM, 50% inhibition | Rhodopseudomonas palustris | |
| 7.1.1.9 | Lithium diiodosalicylate | - |
Rhodopseudomonas palustris | |
| 7.1.1.9 | N,N-Dimethyllauryl amine oxide | - |
Rhodopseudomonas palustris | |
| 7.1.1.9 | phosphate | more than 10 mM | Rhodopseudomonas palustris | |
| 7.1.1.9 | sodium deoxycholate | - |
Rhodopseudomonas palustris | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.1.1.9 | 0.019 | - |
ferrocytochrome c | oxidation of horse cytochrome c | Rhodopseudomonas palustris | |
| 7.1.1.9 | 0.026 | - |
ferrocytochrome c | oxidation of bovine or yeast cytochrome c | Rhodopseudomonas palustris | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 7.1.1.9 | 9500 | - |
x * 30500 + x * 25500 + x * 12200 + x * 9500, SDS-PAGE | Rhodopseudomonas palustris |
| 7.1.1.9 | 12200 | - |
x * 30500 + x * 25500 + x * 12200 + x * 9500, SDS-PAGE | Rhodopseudomonas palustris |
| 7.1.1.9 | 25500 | - |
x * 30500 + x * 25500 + x * 12200 + x * 9500, SDS-PAGE | Rhodopseudomonas palustris |
| 7.1.1.9 | 30500 | - |
x * 30500 + x * 25500 + x * 12200 + x * 9500, SDS-PAGE | Rhodopseudomonas palustris |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.1.1.9 | Rhodopseudomonas palustris | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 7.1.1.9 | - |
Rhodopseudomonas palustris |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 7.1.1.9 | 50.7 | - |
oxidation of horse heart cytochrome c | Rhodopseudomonas palustris |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.1.9 | ferrocytochrome c + O2 + H+ | - |
Rhodopseudomonas palustris | ferricytochrome c + H2O | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.1.1.9 | ? | x * 30500 + x * 25500 + x * 12200 + x * 9500, SDS-PAGE | Rhodopseudomonas palustris |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 7.1.1.9 | 5.5 | 6 | glycylglycine/NaOH buffer | Rhodopseudomonas palustris |
| 7.1.1.9 | 6 | - |
phosphate buffer | Rhodopseudomonas palustris |
| 7.1.1.9 | 7.5 | 8 | presence of reducing agents | Rhodopseudomonas palustris |
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