Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Qian, J.; Shi, W.; Pressler, M.; Hoganson, C.; Mills, D.; Babcock, G.T.; Ferguson-Miller, S.
    Aspartate-407 in Rhodobacter sphaeroides cytochrome c oxidase is not required for proton pumping or manganese binding (1997), Biochemistry, 36, 2539-2543.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.9 overexpression of wild-type, D407A, D407C and D407N mutant enzyme in Rhodobacter sphaeroides Cereibacter sphaeroides

Protein Variants

EC Number Protein Variants Comment Organism
7.1.1.9 D407A similar properties as wild type, suggesting that D407 does not play a role in proton translocating Cereibacter sphaeroides
7.1.1.9 D407C similar properties as wild type, suggesting that D407 does not play a role in proton translocating Cereibacter sphaeroides
7.1.1.9 D407N similar properties as wild type, suggesting that D407 does not play a role in proton translocating Cereibacter sphaeroides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.1.1.9 Cu 2 copper ions at the CuA site Cereibacter sphaeroides
7.1.1.9 Mg
-
 Cereibacter sphaeroides
7.1.1.9 Mn when grown with 0.7 mM Mn2+ and 0.05 mM Mg2+, Mn2+ appears to be inserted into what is normally a Mg2+ site Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.9 Cereibacter sphaeroides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.1.1.9 recombinant wild-type, D407A, D407C and D407N mutant enzyme, Ni2+-nitrilotriacetic acid affinity chromatography Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.9 ferrocytochrome c + O2 + H+
-
 Cereibacter sphaeroides ferricytochrome c + H2O
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1.1.9 550
-
 ferrocytochrome c reconstituted recombinant D407C mutant enzyme Cereibacter sphaeroides
7.1.1.9 650
-
 ferrocytochrome c recombinant D407C mutant enzyme Cereibacter sphaeroides
7.1.1.9 700
-
 ferrocytochrome c reconstituted recombinant D407A mutant enzyme Cereibacter sphaeroides
7.1.1.9 700
-
 ferrocytochrome c reconstituted recombinant D407N mutant enzyme Cereibacter sphaeroides
7.1.1.9 1100
-
 ferrocytochrome c recombinant D407A mutant enzyme Cereibacter sphaeroides
7.1.1.9 1200
-
 ferrocytochrome c reconstituted wild-type enzyme Cereibacter sphaeroides
7.1.1.9 1300
-
 ferrocytochrome c wild-type enzyme Cereibacter sphaeroides
7.1.1.9 1400
-
 ferrocytochrome c recombinant D407N mutant enzyme Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1.1.9 6.5
-
 wild-type, D407A, D407C and D407N mutant enzyme, sharp drop above Cereibacter sphaeroides