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Literature summary extracted from

  • Zhen, Y.; Hoganson, C.W.; Babcock, G.T.; Ferguson-Miller, S.
    Definition of the interaction domain for cytochrome c on cytochrome c oxidase. I. Biochemical, spectral, and kinetic characterization of surface mutants in subunit ii of Rhodobacter sphaeroides cytochrome aa(3) (1999), J. Biol. Chem., 274, 38032-38041.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.1.1.9 overexpression of wild-type, E148Q, D195N, E157Q, D214N, D188Q/E189N, D151Q/E152N, W143F, W143A and Y144A/W145A in Rhodobacter sphaeroides Cereibacter sphaeroides

Protein Variants

EC Number Protein Variants Comment Organism
7.1.1.9 D151Q/E152N similar steady-state kinetic as wild-type Cereibacter sphaeroides
7.1.1.9 D188Q/E189N similar steady-state kinetic as wild-type Cereibacter sphaeroides
7.1.1.9 D195N decrease in activity, increase in Km Cereibacter sphaeroides
7.1.1.9 D214N decrease in activity, increase in Km Cereibacter sphaeroides
7.1.1.9 E148Q decrease in activity, increase in Km Cereibacter sphaeroides
7.1.1.9 E157Q decrease in activity, increase in Km Cereibacter sphaeroides
7.1.1.9 W143A similar copper/Fe ratios as wild-type Cereibacter sphaeroides
7.1.1.9 W143F similar copper/Fe ratios as wild-type Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.1.1.9 additional information
-
additional information comparison of high and low affinity Km values at low ionic strength with Rhodobacter spaeroides and horse cytochrome c of wild-type and various mutants Cereibacter sphaeroides

Organism

EC Number Organism UniProt Comment Textmining
7.1.1.9 Cereibacter sphaeroides
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.1.1.9 ferrocytochrome c + O2 + H+
-
Cereibacter sphaeroides ferricytochrome c + H2O
-
r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1.1.9 additional information
-
additional information highest turnover number at 55 mM ionic strength: D214N, D195N and E148Q, highest turnover number at 45 mM ionic strength: E157Q, comparison of high and low affinity turnover numbers at low ionic strength with Rhodobacter spaeroides and horse cytochrome c of wild-type and various mutants Cereibacter sphaeroides
7.1.1.9 6
-
ferrocytochrome c Rhodobacter sphaeroides cytochrome c, W143A mutant enzyme Cereibacter sphaeroides
7.1.1.9 20
-
ferrocytochrome c horse cytochrome c, W143A mutant enzyme Cereibacter sphaeroides
7.1.1.9 20
-
ferrocytochrome c Rhodobacter sphaeroides cytochrome c, W143F mutant enzyme Cereibacter sphaeroides
7.1.1.9 40
-
ferrocytochrome c horse cytochrome c, W143F mutant enzyme Cereibacter sphaeroides
7.1.1.9 50
-
ferrocytochrome c Rhodobacter sphaeroides cytochrome c, D229N mutant enzyme Cereibacter sphaeroides
7.1.1.9 54
-
ferrocytochrome c Rhodobacter sphaeroides cytochrome c, D195Q mutant enzyme Cereibacter sphaeroides
7.1.1.9 100
-
ferrocytochrome c Rhodobacter sphaeroides cytochrome c, E157Q and E254A mutant enzyme Cereibacter sphaeroides
7.1.1.9 180
-
ferrocytochrome c Rhodobacter sphaeroides cytochrome c, D214N mutant enzyme Cereibacter sphaeroides
7.1.1.9 220
-
ferrocytochrome c Rhodobacter sphaeroides cytochrome c, D151N/E152Q mutant enzyme Cereibacter sphaeroides
7.1.1.9 230
-
ferrocytochrome c Rhodobacter sphaeroides cytochrome c, E148Q mutant enzyme Cereibacter sphaeroides
7.1.1.9 260
-
ferrocytochrome c Rhodobacter sphaeroides cytochrome c, D188N/E189Q mutant enzyme Cereibacter sphaeroides
7.1.1.9 270
-
ferrocytochrome c Rhodobacter sphaeroides cytochrome c, wild-type enzyme Cereibacter sphaeroides
7.1.1.9 290
-
ferrocytochrome c wild-type enzyme, horse cytochrome c, 155 mM ionic strength Cereibacter sphaeroides
7.1.1.9 400
-
ferrocytochrome c horse cytochrome c, D214N and D229N mutant enzyme Cereibacter sphaeroides
7.1.1.9 590
-
ferrocytochrome c wild-type enzyme, horse cytochrome c, 5 mM ionic strength Cereibacter sphaeroides
7.1.1.9 600
-
ferrocytochrome c horse cytochrome c, E157Q and E254Amutant enzyme Cereibacter sphaeroides
7.1.1.9 980
-
ferrocytochrome c wild-type, D188N/E189Q and D151N/E152Q mutant enzyme, horse cytochrome c, 75 mM ionic strength Cereibacter sphaeroides
7.1.1.9 1100
-
ferrocytochrome c horse cytochrome c, E148Q mutant enzyme Cereibacter sphaeroides
7.1.1.9 1200
-
ferrocytochrome c horse cytochrome c, D195Q mutant enzyme Cereibacter sphaeroides
7.1.1.9 1300
-
ferrocytochrome c horse cytochrome c, D151N/E152Q mutant enzyme Cereibacter sphaeroides
7.1.1.9 1600
-
ferrocytochrome c horse cytochrome c, D188N/E189Q mutant enzyme Cereibacter sphaeroides
7.1.1.9 1700
-
ferrocytochrome c horse cytochrome c, wild-type enzyme Cereibacter sphaeroides