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Literature summary extracted from

  • Hughes, R.K.; West, S.I.; Hornostaj, A.R.; Lawson, D.M.; Fairhurst, S.A.; Sanchez, R.O.; Hough, P.; Robinson, B.H.; Casey, R.
    Probing a novel potato lipoxygenase with dual positional specificity reveals primary determinants of substrate binding and requirements for a surface hydrophobic loop and has implications for the role of lipoxygenases in tubers (2001), Biochem. J., 353, 345-355.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.B6 expression in Escherichia col BL21(DE3) Solanum tuberosum

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.B6 I437L KM-value for linoleate is 114% of wild-type value, kcat/Km for linoleate is 83% of wild-type value. Positional specificity 13-hydroperoxy-(10E,12Z)-octadecadienoate/9-hydroperoxy-(10E,12Z)-octadecadienoate is 0.7 (compared to 0.8 in reaction with wild-type enzyme) Solanum tuberosum
1.13.11.B6 V570I KM-value for linoleate is 61% of wild-type value, kcat/Km for linoleate is 367% of wild-type value. Positional specificity 13-hydroperoxy-(10E,12Z)-octadecadienoate/9-hydroperoxy-(10E,12Z)-octadecadienoate is 0.6 (compared to 0.8 in reaction with wild-type enzyme) Solanum tuberosum
1.13.11.B6 V580F KM-value for linoleate is 384% of wild-type value, kcat/Km for linoleate is 4% of wild-type value. Positional specificity 13-hydroperoxy-(10E,12Z)-octadecadienoate/9-hydroperoxy-(10E,12Z)-octadecadienoate is 0.9 (compared to 0.8 in reaction with wild-type enzyme) Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.B6 0.2
-
 linoleate pH 5.5, 25°C Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.B6 Solanum tuberosum Q9SC16 cv. Desiree
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.B6
-
 Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.B6 tuber potato 13/9-LOX is only a minor component of the total LOX population in tubers Solanum tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.B6 65
-
-
 Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.B6 linoleate + O2
-
 Solanum tuberosum (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate dual positional specificity: the enzyme produces roughly equimolar amounts of (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate and (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate. The R/S stereoconfiguration of the products is not determined ?
1.13.11.B6 additional information the enzyme catalyzes oxidation of arachidonate with lower activity Solanum tuberosum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.B6 13/9 lipoxygenase
-
 Solanum tuberosum
1.13.11.B6 13/9-LOX
-
 Solanum tuberosum
1.13.11.B6 lox1-St-2
-
 Solanum tuberosum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.B6 106
-
 linoleate pH 5.5, 25°C Solanum tuberosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.B6 5.5
-
-
 Solanum tuberosum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.B6 4.5 7 pH 4.5: about 45% of maximal activity, pH 7.0: about 55% of maximal activity Solanum tuberosum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.B6 530
-
 linoleate pH 5.5, 25°C Solanum tuberosum