Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zouari, N.; Romette, J.L.; Thomas, D.
    Purification and properties of two laccase isoenzymes produced by Botrytis cinerea (1987), Appl. Biochem. Biotechnol., 15, 213-225.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 azide
-
Botrytis cinerea
1.10.3.2 CN-
-
Botrytis cinerea
1.10.3.2 diethyldithiocarbamate
-
Botrytis cinerea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.133
-
O2 laccase 1 Botrytis cinerea
1.10.3.2 0.533
-
O2 laccase 2 Botrytis cinerea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 72400
-
x * 72400, SDS-PAGE, probably anomalous migration on SDS-gels Botrytis cinerea
1.10.3.2 96000 110000 gel filtration, electrophoresis results in 72400 Da Botrytis cinerea

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Botrytis cinerea
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.2 glycoprotein sugar content laccase 1: 86%, laccase 2: 91% Botrytis cinerea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 1.66 28.67 micromol O2/min/mg depending on organism and isozyme Botrytis cinerea

Storage Stability

EC Number Storage Stability Organism
1.10.3.2 -20°C, several months stable Botrytis cinerea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 1,2,4-benzenetriol + O2
-
Botrytis cinerea ?
-
?
1.10.3.2 1-naphthol + O2
-
Botrytis cinerea ? + H2O
-
?
1.10.3.2 2 catechol + O2
-
Botrytis cinerea 2 6-hydroxycyclohexa-2,4-dienone + 2 H2O
-
?
1.10.3.2 2-naphthol + O2
-
Botrytis cinerea ? + H2O
-
?
1.10.3.2 3,5-dimethoxy-hydroxy-benzaldazine + O2
-
Botrytis cinerea ? + H2O
-
?
1.10.3.2 4,5-dimethyl-o-phenylenediamine + O2
-
Botrytis cinerea ? + H2O
-
?
1.10.3.2 4-amino-N,N'-dimethylaniline + O2
-
Botrytis cinerea ? + H2O
-
?
1.10.3.2 4-methylcatechol + O2
-
Botrytis cinerea 6-hydroxy-4-methylcyclohexa-2,4-dienone + H2O
-
?
1.10.3.2 ascorbate + O2
-
Botrytis cinerea ?
-
?
1.10.3.2 caffeic acid + O2
-
Botrytis cinerea ?
-
?
1.10.3.2 gallic acid + O2
-
Botrytis cinerea ?
-
?
1.10.3.2 guaiacol + O2
-
Botrytis cinerea 6-methoxycyclohexa-2,4-dienone + H2O
-
?
1.10.3.2 additional information overview Botrytis cinerea ?
-
?
1.10.3.2 o-cresol + O2
-
Botrytis cinerea 6-hydroxy-2-methylcyclohexa-2,4-dienone + H2O
-
?
1.10.3.2 p-cresol + O2
-
Botrytis cinerea 6-hydroxy-4-methylcyclohexa-2,4-dienone + H2O
-
?
1.10.3.2 p-phenylenediamine + O2
-
Botrytis cinerea ? + H2O
-
?
1.10.3.2 pyrogallol + O2
-
Botrytis cinerea ?
-
?
1.10.3.2 quinol + O2
-
Botrytis cinerea ? + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 oligomer x * 72400, SDS-PAGE, probably anomalous migration on SDS-gels Botrytis cinerea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 55
-
laccase 1 Botrytis cinerea
1.10.3.2 60
-
laccase 2 Botrytis cinerea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 additional information
-
laccase 2 has higher thermostability than laccase 1 Botrytis cinerea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 2.5 3 laccase 1 Botrytis cinerea
1.10.3.2 4
-
laccase 2 Botrytis cinerea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 5
-
decline of activity above Botrytis cinerea