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Literature summary extracted from

  • Saito, K.; Komamine, A.
    Biosynthesis of stizolobinic acid and stizolobic acid in higher plants (1978), Eur. J. Biochem., 82, 385-392.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.13.11.29 DTT stabilizes Stizolobium hassjoo
1.13.11.30 DTT stabilizes Stizolobium hassjoo

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.29 8-Hydroxyquinoline-5-sulfonate 90% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.29 Al3+ 95% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.29 alpha,alpha'-dipyridyl
-
 Stizolobium hassjoo
1.13.11.29 Co2+ 65% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.29 Cu2+ 85% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.29 Fe2+ 85% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.29 Fe3+ 90% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.29 glutathione 55% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.29 iodoacetate 55% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.29 KCN
-
 Stizolobium hassjoo
1.13.11.29 Mg2+ 65% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.29 Mn2+ 95% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.29 Mo6+ 65% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.29 N-ethylmaleimide 45% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.29 Neocuproine 55% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.29 o-phenanthroline 85% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.29 p-chloromercuribenzoate 80% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.29 Salicylaldoxime 60% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.30 8-Hydroxyquinoline-5-sulfonate 90% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.30 Al3+ 95% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.30 alpha,alpha'-dipyridyl 70% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.30 Co2+ 85% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.30 Cu2+ 85% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.30 Fe2+ 75% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.30 Fe3+ 95% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.30 glutathione 70% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.30 iodoacetate 55% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.30 KCN 85% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.30 Mg2+ 65% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.30 Mn2+ 95% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.30 Mo6+ 80% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.30 N-ethylmaleimide 35% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.30 Neocuproine 50% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.30 o-phenanthroline 90% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.30 p-chloromercuribenzoate 70% inhibition at 0.1 mM Stizolobium hassjoo
1.13.11.30 Salicylaldoxime 65% inhibition at 0.1 mM Stizolobium hassjoo

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.29 0.00022
-
 NADP+
-
 Stizolobium hassjoo
1.13.11.29 1.39
-
 3,4-dihydroxyphenylalanine
-
 Stizolobium hassjoo
1.13.11.30 0.00026
-
 NADP+
-
 Stizolobium hassjoo
1.13.11.30 1.67
-
 3,4-dihydroxyphenylalanine
-
 Stizolobium hassjoo

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.29 Zn2+ activation Stizolobium hassjoo
1.13.11.30 Zn2+ activation Stizolobium hassjoo

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.29 45000
-
 gel filtration Stizolobium hassjoo
1.13.11.30 45000
-
 gel filtration Stizolobium hassjoo

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.29 3,4-dihydroxy-L-phenylalanine + O2 Stizolobium hassjoo
-
 4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
-
 ?
1.13.11.30 3,4-dihydroxy-L-phenylalanine + O2 Stizolobium hassjoo
-
 5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.29 Stizolobium hassjoo
-
-
-
1.13.11.30 Stizolobium hassjoo
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.29
-
 Stizolobium hassjoo
1.13.11.30
-
 Stizolobium hassjoo

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.29 seedling etiolated Stizolobium hassjoo
-
1.13.11.30 seedling
-
 Stizolobium hassjoo
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.29 0.06
-
-
 Stizolobium hassjoo
1.13.11.30 0.031
-
-
 Stizolobium hassjoo

Storage Stability

EC Number Storage Stability Organism
1.13.11.29 -20°C, 0.1 mM DTT, stable for 3 months Stizolobium hassjoo
1.13.11.29 30°C, half-life of 3-5 h Stizolobium hassjoo
1.13.11.30 -20°C, 0.1 mM DTT, nitrogen atmosphere, stable for 3 months Stizolobium hassjoo
1.13.11.30 30°C, purified enzyme, half life of 3-5 h Stizolobium hassjoo

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.29 3,4-dihydroxy-L-phenylalanine + O2
-
 Stizolobium hassjoo 4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
-
 ?
1.13.11.29 3,4-dihydroxy-L-phenylalanine + O2
-
 Stizolobium hassjoo 4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde undergoes ring closure and oxidation to stizolobate with NAD(P)+ as acceptor ?
1.13.11.29 4-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+
-
 Stizolobium hassjoo stizolobate + NADPH + H+
-
 r
1.13.11.30 3,4-dihydroxy-L-phenylalanine + O2
-
 Stizolobium hassjoo 5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde
-
 ?
1.13.11.30 3,4-dihydroxy-L-phenylalanine + O2
-
 Stizolobium hassjoo 5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde undergoes ring closure and oxidation to stizolobinate with NAD(P)+ as acceptor ?
1.13.11.30 5-(L-alanin-3-yl)-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+
-
 Stizolobium hassjoo stizolobinate + NADPH + H+
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.29 7.4
-
-
 Stizolobium hassjoo
1.13.11.30 7.6
-
-
 Stizolobium hassjoo

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.11.29 NAD+ required for reaction, less efficient than NADP+ Stizolobium hassjoo
1.13.11.29 NADP+ required for reaction Stizolobium hassjoo
1.13.11.30 NAD+ required for reaction, less efficient than NADP+ Stizolobium hassjoo
1.13.11.30 NADP+ required for reaction Stizolobium hassjoo