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Literature summary extracted from

  • Saunders, P.P.; Broquist, H.P.
    Saccharopine, an intermediate of the aminoadipic acid pathway of lysine biosynthesis. IV. Saccharopine dehydrogenase (1966), J. Biol. Chem., 241, 3435-3440.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.1.7 2-mercaptoethanol, 1 mM, stabilizes Saccharomyces cerevisiae
1.5.1.7 high salt concentration stabilizes Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.7 iodoacetate not Saccharomyces cerevisiae
1.5.1.7 p-hydroxymercuribenzoate NAD+ protects Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.7 0.44
-
 2-oxoglutarate
-
 Saccharomyces cerevisiae
1.5.1.7 0.46
-
 NADH
-
 Saccharomyces cerevisiae
1.5.1.7 12
-
 L-lysine
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.7 49000
-
 sucrose density gradient centrifugation Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.7 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O Saccharomyces cerevisiae involved in lysine biosynthesis L-lysine + 2-oxoglutarate + NADH
-
 ?
1.5.1.7 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O Saccharomyces cerevisiae last step of alpha-aminoadipate pathway for lysine biosynthesis L-lysine + 2-oxoglutarate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.7 Saccharomyces cerevisiae
-
 baker's yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.7
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.7 additional information
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.7 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O high specificity with respect to coenzyme and substrate Saccharomyces cerevisiae L-lysine + 2-oxoglutarate + NADH
-
 r
1.5.1.7 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O i.e. saccharopine Saccharomyces cerevisiae L-lysine + 2-oxoglutarate + NADH
-
 r
1.5.1.7 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O involved in lysine biosynthesis Saccharomyces cerevisiae L-lysine + 2-oxoglutarate + NADH
-
 ?
1.5.1.7 N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O last step of alpha-aminoadipate pathway for lysine biosynthesis Saccharomyces cerevisiae L-lysine + 2-oxoglutarate + NADH
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.7 25
-
 assay at Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.7 18.8
-
 NADH
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.7 7
-
 L-lysine + 2-oxoglutarate + NADH Saccharomyces cerevisiae
1.5.1.7 10
-
 or higher, saccharopine + NAD+ + H2O Saccharomyces cerevisiae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.7 5.2 9 half-maximal activities at pH 5.2 and 9.0, L-lysine + 2-oxoglutarate + NADH Saccharomyces cerevisiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.5.1.7 5 8
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.7 NAD+
-
 Saccharomyces cerevisiae