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Literature summary extracted from

  • Iwaki, M.; Yagi, T.; Horiike, K.; Saeki, Y.; Ushijima, T.; Nozaki, M.
    Crystallization and properties of aromatic amine dehydrogenase from Pseudomonas sp (1983), Arch. Biochem. Biophys., 220, 253-262.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.9.2
-
 Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.9.2 beta-phenylethylamine substrate inhibition Pseudomonas sp.
1.4.9.2 hydroxylamine
-
 Pseudomonas sp.
1.4.9.2 phenylhydrazine
-
 Pseudomonas sp.
1.4.9.2 Semicarbazide
-
 Pseudomonas sp.
1.4.9.2 tryptamine substrate inhibition Pseudomonas sp.
1.4.9.2 tyramine substrate inhibition Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.9.2 0.0012
-
 tyramine
-
 Pseudomonas sp.
1.4.9.2 0.0045
-
 2-Phenylethylamine
-
 Pseudomonas sp.
1.4.9.2 0.0092
-
 tryptamine
-
 Pseudomonas sp.
1.4.9.2 0.11
-
 phenazine methosulfate
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.9.2 copper metal content: 0.1 mol per mol of enzyme Pseudomonas sp.
1.4.9.2 Iron metal content: 0.6 mol per mol of enzyme Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.9.2 8000
-
 alpha2beta2, 2 * 46000 + 2 * 8000, SDS-PAGE Pseudomonas sp.
1.4.9.2 46000
-
 alpha2beta2, 2 * 46000 + 2 * 8000, SDS-PAGE Pseudomonas sp.
1.4.9.2 103000
-
 sedimentation equilibrium Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.4.9.2 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.9.2
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.9.2 9.11
-
 after purification Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.9.2 2-phenylethylamine + H2O + 2,6-dichlorophenolindophenol
-
 Pseudomonas sp. phenylacetaldehyde + NH3 + reduced 2,6-dichlorophenolindophenol
-
 r
1.4.9.2 dopamine + acceptor + H2O
-
 Pseudomonas sp. 4-(2-oxoethyl)-1,2-benzenediol + NH3 + reduced acceptor
-
 ?
1.4.9.2 additional information long-chain aliphatic amines, phenazine methosulfate is the only effective electron acceptor Pseudomonas sp. ?
-
 ?
1.4.9.2 additional information not: methylamine, ethylamine, polyamines Pseudomonas sp. ?
-
 ?
1.4.9.2 additional information slow reaction with aromatic amines Pseudomonas sp. ?
-
 ?
1.4.9.2 n-hexylamine + acceptor + H2O
-
 Pseudomonas sp. hexanal + NH3 + reduced acceptor
-
 ?
1.4.9.2 n-octylamine + acceptor + H2O
-
 Pseudomonas sp. octanal + NH3 + reduced acceptor
-
 ?
1.4.9.2 phenazine methosulfate + H2O + 2,6-dichlorophenol indophenol
-
 Pseudomonas sp. ? + NH3 + reduced 2,6-dichlorophenol indophenol
-
 ?
1.4.9.2 serotonin + acceptor + H2O
-
 Pseudomonas sp. 3-(2-oxoethyl)-1H-indol-5-ol + NH3 + reduced acceptor
-
 ?
1.4.9.2 tryptamine + acceptor + H2O
-
 Pseudomonas sp. 1H-indole-3-acetaldehyde + NH3 + reduced acceptor
-
 ?
1.4.9.2 tyramine + acceptor + H2O
-
 Pseudomonas sp. 4-hydroxyphenylacetaldehyde + NH3 + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.9.2 tetramer alpha2beta2, 2 * 46000 + 2 * 8000, SDS-PAGE Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.9.2 7.5
-
 tyramine, beta-phenylethylamine Pseudomonas sp.
1.4.9.2 8
-
 serotonin, tryptamine Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.9.2 5.5 9.5 5.5: about 25% of activity maximum, 9.5: about 10% of activity maximum Pseudomonas sp.